Comparative Analyses of Phytochemical Variation Within and Between Congeneric Species of Willow Herb, Epilobium hirsutum and E. parviflorum: Contribution of Environmental Factors
Open Access
- 17 February 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Plant Science
Abstract
The plants in the Epilobium genus are considered to have several important medicinal properties due to their unique chemical composition. Although metabolic profiles of medicinal plants are mainly controlled by genetic factors, their production is also to some degree influenced by environmental factors, thus, variations in the levels of phytochemicals may represent long-term ecological and evolutionary interactions. In order to depict the magnitude of natural variation in level of chemical compounds among conspecific populations of Epilobium hirsutum (n = 31) and E. parviflorum (n = 16), metabolite profiling of aerial parts of plants was performed with gas chromatography/mass spectrometry analysis. Putative identification and structure annotation revealed the presence of 74 compounds including 46 compounds considered secondary metabolites categorized into flavonoids (n = 8), phenolic acids (n = 26), steroids (n = 3), and terpenes (n = 5) across all populations. Although there was a considerable natural variation among conspecific populations, principal component analysis revealed a clear separation of populations of each species based on the second main principal component which was highly correlated with eight secondary metabolites. The level of secondary metabolites was significantly correlated between species (r = 0.91), suggesting shared metabolic pathways underlying the production of chemical compounds. In addition, redundancy and variance partitioning analyses by including bioclimatic variables and altitude revealed a significant contribution of elevation in explaining the total variation of secondary metabolites in E. hirsutum. Two-thirds of all secondary metabolites were significantly correlated with altitude in E. hirsutum. The large-scale geographic analyses of populations revealed additionally detected flavonoids and terpenes (E. hirsutum and E. parviflorum) and steroids (E. hirsutum) for the first time. This study provides significant information on additional chemical compounds found across the distribution range of the two ecologically important species of willow herb and emphasizes the importance of geographic-wide sampling as a valuable strategy to depict intraspecific and interspecific variability in chemical traits.Keywords
Funding Information
- Iran National Science Foundation (92034833)
This publication has 45 references indexed in Scilit:
- Ethnopharmacological in vitro studies on Austria's folk medicine—An unexplored lore in vitro anti-inflammatory activities of 71 Austrian traditional herbal drugsJournal of Ethnopharmacology, 2013
- Extracts from Epilobium sp. herbs induce apoptosis in human hormone-dependent prostate cancer cells by activating the mitochondrial pathwayJournal of Pharmacy and Pharmacology, 2013
- Effect of latitude on flavonoid biosynthesis in plantsPlant, Cell & Environment, 2010
- Improved and validated HPTLC method for quantification of oenothein B and its use for analysis ofEpilobiumangustifoliumL.Journal of Planar Chromatography – Modern TLC, 2010
- Polyphenol composition and antioxidant capacity of Epilobium speciesJournal of Pharmaceutical and Biomedical Analysis, 2009
- Densitometric determination of flavonoids in methanolic and aqueous extracts ofEpilobii angustifolii herbaby use of HPTLCJournal of Planar Chromatography – Modern TLC, 2007
- Identification of Epilobium Species and Willow-herbs (Onagraceae) by HPLC Analysis of Flavonoids as Chemotaxonomic MarkersChromatographia, 2006
- Compounds fromEpilobium angustifoliumInhibit the Specific Metallopeptidases ACE, NEP and APNPlanta Medica, 2004
- Determination of Oenothein B as the Active 5-α-Reductase-Inhibiting Principle of the Folk Medicine Epilobium parviflorumJournal of Natural Products, 1996
- Ethnobotany of Costanoan Indians, California, based on collections by John P. HarringtonEconomic Botany, 1984