Differentiated adipose‐derived stem cells promote peripheral nerve regeneration

Abstract

Introduction Introduction: Many reports have indicated that adipose‐derived stem cells (ADSCs) are effective for nerve regeneration. We investigated nerve regeneration by combining a polyglycolic acid‐collagen (PGA‐c) tube, which is approved for clinical use, and Schwann cell‐like differentiated ADSCs (dADSCs). Methods 15‐mm‐long gaps in the sciatic nerve of rats were bridged in each group with tubes (group I), with tubes injected with dADSCs (group II), or by resected nerve (group III). Methods Results: Axonal outgrowth was greater in group II than in group I. Tibialis anterior muscle weight revealed recovery only in group III. Latency in nerve conduction studies was equivalent in group II and III, but action potential was lower in group II. Transplanted dADSCs maintained Schwann cell marker expression. ATF3 expression level in dorsal root ganglia was equivalent in groups II and III. Discussion dADSCs maintained their differentiated state in the tubes and are believed to have contributed to nerve regeneration.