Rapid quantification of fatty acids in plant oils and biological samples by LC-MS
Open Access
- 22 July 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 413 (21), 5439-5451
- https://doi.org/10.1007/s00216-021-03525-y
Abstract
Analysis of fatty acids (FA) in food and biological samples such as blood is indispensable in modern life sciences. We developed a rapid, sensitive and comprehensive method for the quantification of 41 saturated and unsaturated fatty acids by means of LC-MS. Optimized chromatographic separation of isobaric analytes was carried out on a C8 reversed phase analytical column (100 × 2.1 mm, 2.6 μm core–shell particle) with a total run time of 15 min with back pressure lower than 300 bar. On an old triple quadrupole instrument (3200, AB Sciex), pseudo selected reaction monitoring mode was used for quantification of the poorly fragmenting FA, yielding limits of detection of 5–100 nM. Sample preparation was carried out by removal of phospholipids and triglycerides by solid-phase extraction (non-esterified fatty acids in oils) or saponification in iso-propanol (fatty acyls). This is not only a rapid strategy for quantification of fatty acyls, but allows the direct combination with the LC-MS-based analysis of fatty acid oxidation products (eicosanoids and other oxylipins) from the same sample. The concentrations of fatty acyls determined by means of LC-MS were consistent with those from GC-FID analysis demonstrating the accuracy of the developed method. Moreover, the method shows high precisions with a low intra-day (≤ 10% for almost all fatty acids in plasma and ≤ 15% in oils) and inter-day as well as inter-operator variability (< 20%). The method was successfully applied on human plasma and edible oils. The possibility to quantify non-esterified fatty acids in samples containing an excess of triacylglycerols and phospholipids is a major strength of the described approach allowing to gain new insights in the composition of biological samples. Graphical abstractKeywords
Funding Information
- Bergische Universität Wuppertal
This publication has 41 references indexed in Scilit:
- Incorporation of eicosapentaenoic and docosahexaenoic acids into lipid pools when given as supplements providing doses equivalent to typical intakes of oily fishThe American Journal of Clinical Nutrition, 2012
- Nonesterified Fatty Acid Determination for Functional Lipidomics: Comprehensive Ultrahigh Performance Liquid Chromatography–Tandem Mass Spectrometry Quantitation, Qualification, and Parameter PredictionAnalytical Chemistry, 2012
- The Human Plasma LipidomeThe New England Journal of Medicine, 2011
- Characterization of fatty acid and triacylglycerol composition in animal fats using silver-ion and non-aqueous reversed-phase high-performance liquid chromatography/mass spectrometry and gas chromatography/flame ionization detectionJournal of Chromatography A, 2011
- Improved LC−MS Method for the Determination of Fatty Acids in Red Blood Cells by LC−Orbitrap MSAnalytical Chemistry, 2011
- Thematic Review Series: Proteomics. An integrated omics analysis of eicosanoid biologyJournal of Lipid Research, 2009
- Plasma free fatty acid profiling in a fish oil human intervention study using ultra‐performance liquid chromatography/electrospray ionization tandem mass spectrometryRapid Communications in Mass Spectrometry, 2008
- Mechanisms linking obesity to insulin resistance and type 2 diabetesNature, 2006
- Endocannabinoid metabolomics: A novel liquid chromatography-mass spectrometry reagent for fatty acid analysisThe AAPS Journal, 2006
- A Gas Chromatography/Electron Ionization−Mass Spectrometry−Selected Ion Monitoring Method for Determining the Fatty Acid Pattern in Food after Formation of Fatty Acid Methyl EstersJournal of Agricultural and Food Chemistry, 2005