Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions
Open Access
- 1 March 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Cell and Developmental Biology
Abstract
Glucose is a major energy source consumed by proliferating mammalian cells. Therefore, in general, proliferating cells have the preference of high glucose contents in extracellular environment. Here, we showed that high glucose concentrations impede the proliferation of satellite cells, which are muscle-specific stem cells, under adherent culture conditions. We found that the proliferation activity of satellite cells was higher in glucose-free DMEM growth medium (low-glucose medium with a glucose concentration of 2 mM) than in standard glucose DMEM (high-glucose medium with a glucose concentration of 19 mM). Satellite cells cultured in the high-glucose medium showed a decreased population of reserve cells, identified by staining for Pax7 expression, suggesting that glucose concentration affects cell fate determination. In conclusion, glucose is a factor that decides the cell fate of skeletal muscle-specific stem cells. Due to this unique feature of satellite cells, hyperglycemia may negatively affect the regenerative capability of skeletal muscle myofibers and thus facilitate sarcopenia.Keywords
Funding Information
- Japan Society for the Promotion of Science (KAKENHI 18K19751, KAKENHI 20H04079, KAKENHI 17H02159, KAKENHI 18H04086)
This publication has 39 references indexed in Scilit:
- Low Glucose but Not Galactose Enhances Oxidative Mitochondrial Metabolism in C2C12 Myoblasts and MyotubesPLOS ONE, 2013
- Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal MuscleJournal of Visualized Experiments, 2013
- Tissue-Specific Stem Cells: Lessons from the Skeletal Muscle Satellite CellCell Stem Cell, 2012
- High Glucose Promotes Pancreatic Cancer Cell Proliferation via the Induction of EGF Expression and Transactivation of EGFRPLOS ONE, 2011
- Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Culture SystemPublished by Springer Science and Business Media LLC ,2011
- Muscle satellite cells are a functionally heterogeneous population in both somite-derived and branchiomeric musclesDevelopmental Biology, 2010
- Self-renewal and expansion of single transplanted muscle stem cellsNature, 2008
- Stem Cell Function, Self-Renewal, and Behavioral Heterogeneity of Cells from the Adult Muscle Satellite Cell NicheCell, 2005
- Pattern of Pax7 expression during myogenesis in the posthatch chicken establishes a model for satellite cell differentiation and renewalDevelopmental Dynamics, 2004
- Screening for Type 2 DiabetesDiabetes Care, 2003