CAP-MAP: cap analysis protocol with minimal analyte processing, a rapid and sensitive approach to analysing mRNA cap structures
Open Access
- 26 February 2020
- journal article
- research article
- Published by The Royal Society in Open Biology
- Vol. 10 (2), 190306
- https://doi.org/10.1098/rsob.190306
Abstract
Eukaryotic messenger RNA (mRNA) is modified by the addition of an inverted guanosine cap to the 5′ triphosphate. The cap guanosine and initial transcribed nucleotides are further methylated by a series of cap methyltransferases to generate the mature cap structures which protect RNA from degradation and recruit proteins involved in RNA processing and translation. Research demonstrating that the cap methyltransferases are regulated has generated interest in determining the methylation status of the mRNA cap structures present in cells. Here, we present CAP-MAP: cap analysis protocol with minimal analyte processing, a rapid and sensitive method for detecting cap structures present in mRNA isolated from tissues or cultured cells.Keywords
Funding Information
- Medical Research Council (MR/K024213/1 Senior Fellowship)
- Lister Institute of Preventive Medicine (Prize Fellowship)
- H2020 European Research Council (769080)
- Wellcome Trust (097945/Z/11/Z)
- Royal Society (Wolfson Research Merit Award)
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