Leukocyte Trafficking and Hemostasis in the Mouse Fetus in vivo: A Practical Guide
Open Access
- 21 January 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Cell and Developmental Biology
Abstract
In vivo observations of blood cells and organ compartments within the fetal mammalian organism are difficult to obtain. This practical guide describes a mouse model for in vivo observation of the fetal yolk-sac and corporal microvasculature throughout murine gestation, including imaging of various organ compartments, microvascular injection procedures, different methods for staining of blood plasma, vessel wall and circulating cell subsets. Following anesthesia of pregnant mice, the maternal abdominal cavity is opened, the uterus horn exteriorized, and the fetus prepared for imaging while still connected to the placenta. Microinjection methods allow delivery of substances directly into the fetal circulation, while substances crossing the placenta can be easily administered via the maternal circulation. Small volume blood sample collection allows for further in vitro workup of obtained results. The model permits observation of leukocyte-endothelial interactions, hematopoietic niche localization, platelet function, endothelial permeability studies, and hemodynamic changes in the mouse fetus, using appropriate strains of fluorescent protein expressing reporter mice and various sophisticated intravital microscopy techniques. Our practical guide is of interest to basic physiologists, developmental biologists, cardiologists, and translational neonatologists and reaches out to scientists focusing on the origin and regulation of hematopoietic niches, thrombopoiesis and macrophage heterogeneity.This publication has 48 references indexed in Scilit:
- Antibodies against neutrophil LY6G do not inhibit leukocyte recruitment in mice in vivoBlood, 2013
- Lineage Tracing of Pf4-Cre Marks Hematopoietic Stem Cells and Their ProgenyPLOS ONE, 2012
- Dynamics of the leading process, nucleus, and Golgi apparatus of migrating cortical interneurons in living mouse embryosProceedings of the National Academy of Sciences of the United States of America, 2012
- Prothrombotic effects of tumor necrosis factor alpha in vivo are amplified by the absence of TNF-alpha receptor subtype 1 and require TNF-alpha receptor subtype 2Arthritis Research & Therapy, 2012
- Two-photon intravital imaging of thrombus developmentJournal of Biomedical Optics, 2010
- Noninvasive high-resolution in vivo imaging of cell biology in the anterior chamber of the mouse eyeNature Protocols, 2008
- The Contribution of Preterm Birth to Infant Mortality Rates in the United StatesPEDIATRICS, 2006
- Targeted insult to subsurface cortical blood vessels using ultrashort laser pulses: three models of strokeNature Methods, 2006
- TheLy‐6A(Sca‐1)GFPTransgene is Expressed in all Adult Mouse Hematopoietic Stem CellsThe International Journal of Cell Cloning, 2002
- Direct Observations of Developing Microcirculatory Patterns in the Posterior Limb Buds of Fetal MiceAngiology, 1961