Effects of supplemental fulvic acid on survival, growth performance, digestive ability and immunity of large yellow croaker (Larimichthys crocea) larvae
Open Access
- 23 March 2023
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Physiology
- Vol. 14, 1159320
- https://doi.org/10.3389/fphys.2023.1159320
Abstract
A 30-day feeding trial was designed to evaluate the effect of supplemental fulvic acid (FA) on survival, growth performance, digestive ability and immunity of large yellow croaker (Larimichthys crocea) larvae (initial body weight 11.33±0.57 mg). Four isonitrogenous and isolipids diets containing 0.00%, 0.01%, 0.02% and 0.04% FA were formulated, respectively. Results showed that the supplementation of 0.04% FA significantly improved survival rate of large yellow croaker larvae. Supplemental FA significantly increased final body weight and specific growth rate. Based on the specific growth rate, the optimal supplementation was 0.0135% FA. Larvae fed the diet with 0.01% FA had significantly higher villus height than the control. The supplementation of 0.01%-0.02% FA significantly increased the muscular thickness of intestine. Moreover, compared with the control, supplementation of FA remarkably increased activities of alkaline phosphatase and leucine aminopeptidase in the brush border membrane of intestine. Diets supplemented with 0.02%-0.04% FA significantly increased the activity of trypsin in the intestinal segment, while 0.01%-0.02% FA significantly increased the activity of trypsin in the pancreatic segment. Meanwhile, supplementation of FA significantly increased mRNA expression of intestinal epithelial proliferation and barrier genes (pcna, zo-1 and zo-2). Furthermore, larvae fed the diet with 0.01% FA significantly increased activities of lysozyme and total nitric oxide synthase. The supplementation of 0.01% to 0.02% FA significantly decreased the mRNA expression of pro-inflammatory cytokines tnf-α and il-6. Concurrently, supplemental FA significantly increased anti-inflammatory cytokine il-10 mRNA expression level. In conclusion, this study indicated that the supplementation of FA could improve the survival rate and growth performance of larvae by promoting intestinal development, digestive enzymes activities and innate immunity.This publication has 54 references indexed in Scilit:
- Effects of dietary phospholipids on survival, growth, digestive enzymes and stress resistance of large yellow croaker, Larmichthys crocea larvaeAquaculture, 2013
- Investigating the biological properties of carbohydrate derived fulvic acid (CHD-FA) as a potential novel therapy for the management of oral biofilm infectionsBMC Oral Health, 2013
- Effects of dietary chitosan and Bacillus subtilis on the growth performance, non-specific immunity and disease resistance of cobia, Rachycentron canadumFish & Shellfish Immunology, 2011
- Optimal dietary lipid level for large yellow croaker (Pseudosciaena crocea) larvaeAquaculture Nutrition, 2008
- Molecular Basis of Epithelial Barrier Regulation: From Basic Mechanisms to Clinical ApplicationThe American Journal of Pathology, 2006
- A histological study on the development of the digestive system of Pseudosciaena crocea larvae and juvenilesJournal of Fish Biology, 2005
- Humic Acid Substances in Animal AgriculturePakistan Journal of Nutrition, 2005
- Activities of selected digestive enzymes during larval development of large yellow croaker (Pseudosciaena crocea)Aquaculture, 2005
- Digestive enzymes in fish larvae and juveniles—implications and applications to formulated dietsAquaculture, 2001
- Topical application of oxifulvic acid suppresses the cutaneous immune response in miceDrug Development Research, 2001