Combinatorial Enzyme Approach to Produce Oligosaccharides of Diverse Structures for Functional Screen
Open Access
- 1 January 2018
- journal article
- Published by Scientific Research Publishing, Inc. in Advances in Enzyme Research
- Vol. 06 (02), 11-20
- https://doi.org/10.4236/aer.2018.62002
Abstract
Combinatorial chemistry has been a focus of research activity in modern drug discovery and biotechnology. It is a concept by which a vast library of molecular diversity is synthesized and screened for target properties. This report is to illustrate the application of enzyme technology using the concept of combinatorial chemistry as a novel approach for the bioconversion of plant fibers. Citrus pectin was subjected to combinatorial enzyme digestion to create libraries of pectic oligosaccharides with diverse structural variants. Repeated cycles of fractionation and screening resulted in the isolation and identification of an active oligoGalA species with antimicrobial activity.Keywords
This publication has 1 reference indexed in Scilit:
- Non-digestible oligosaccharides: A reviewCarbohydrate Polymers, 2007