Metabolic contrast agents produced from transported solid 13C-glucose hyperpolarized via dynamic nuclear polarization

Abstract

Magnetic Resonance Imaging combined with hyperpolarized ¹³C-labelled metabolic contrast agents produced via dissolution Dynamic Nuclear Polarization can, non-invasively and in real-time, report on tissue specific aberrant metabolism. However, hyperpolarization equipment is expensive, technically demanding and needs to be installed on-site for the end-user. In this work, we provide a robust methodology that allows remote production of the hyperpolarized ¹³C-labelled metabolic contrast agents. The methodology, built on photo-induced thermally labile radicals, allows solid sample extraction from the hyperpolarization equipment and several hours’ lifetime of the ¹³C-labelled metabolic contrast agents at appropriate storage/transport conditions. Exemplified with [U-¹³C, d₇]-D-glucose, we remotely produce hyperpolarized ¹³C-labelled metabolic contrast agents and generate above 10,000-fold liquid-state Magnetic Resonance signal enhancement at 9.4 T, keeping on-site only a simple dissolution device.