Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single‐cell profiling
Open Access
- 8 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 40 (6), e105123
- https://doi.org/10.15252/embj.2020105123
Abstract
Similar to the brain, the eye is considered an immune‐privileged organ where tissue‐resident macrophages provide the major immune cell constituents. However, little is known about spatially restricted macrophage subsets within different eye compartments with regard to their origin, function, and fate during health and disease. Here, we combined single‐cell analysis, fate mapping, parabiosis, and computational modeling to comprehensively examine myeloid subsets in distinct parts of the eye during homeostasis. This approach allowed us to identify myeloid subsets displaying diverse transcriptional states. During choroidal neovascularization, a typical hallmark of neovascular age‐related macular degeneration (AMD), we recognized disease‐specific macrophage subpopulations with distinct molecular signatures. Our results highlight the heterogeneity of myeloid subsets and their dynamics in the eye that provide new insights into the innate immune system in this organ which may offer new therapeutic targets for ophthalmological diseases.Keywords
Funding Information
- Deutsche Forschungsgemeinschaft (CRC/TRR167, CRC992, CRC1160, EXC‐2189)
This publication has 75 references indexed in Scilit:
- Bone Marrow Cell Recruitment to the Brain in the Absence of Irradiation or Parabiosis BiasPLOS ONE, 2013
- STAR: ultrafast universal RNA-seq alignerBioinformatics, 2012
- Selective Chemokine Receptor Usage by Central Nervous System Myeloid Cells in CCR2-Red Fluorescent Protein Knock-In MicePLOS ONE, 2010
- Differential expression analysis for sequence count dataGenome Biology, 2010
- Measuring dementia carers' unmet need for services - an exploratory mixed method studyBMC Health Services Research, 2010
- Fast and accurate long-read alignment with Burrows–Wheeler transformBioinformatics, 2010
- DEGseq: an R package for identifying differentially expressed genes from RNA-seq dataBioinformatics, 2009
- Fast and accurate short read alignment with Burrows–Wheeler transformBioinformatics, 2009
- Turnover of bone marrow‐derived cells in the irradiated mouse corneaImmunology, 2008
- The stream of precursors that colonizes the thymus proceeds selectively through the early T lineage precursor stage of T cell developmentThe Journal of Experimental Medicine, 2008