In a retrospective analytic study from NICU, Latifa Woman and Children Hospital - Dubai, UAE. over a period of 12 years (2005 to 2016), 380 neonates and infants had clinical signs of sepsis, confirmed by blood or CSF culture. In very early onset sepsis (< 24 hours), E. Coli represented as the most common organism. In infants with early onset sepsis (2-6 days), Klebsiella Pneumoniae was the most common organism. While in late (7-28 days) & very late onset (29-90 days) the most common organism was Coagulase Negative Staphylococci (CONS) followed by Klebsiella Pneumoniae.