Molecular detection and genotypic characterisation of Streptococcus pneumoniae isolated from children in Malaysia
- 2 January 2020
- journal article
- research article
- Published by Taylor & Francis Ltd in Pathogens and Global Health
- Vol. 114 (1), 46-54
- https://doi.org/10.1080/20477724.2020.1719325
Abstract
Streptococcus pneumoniae (S. pneumoniae) is one of the main causative agents of pneumococcal diseases. To date, more than 90 distinct serotypes have been identified. Implementation of vaccines has caused a drastic reduction in vaccine-serotype pneumococcal diseases but increase in cases due to non-vaccine serotype has been observed in Malaysia. However, further investigation on different serotype incidence in Malaysia is needed and the rate of pneumococcal vaccination for new-born babies in Malaysia remains low. The recent emergence of drug-resistant S. pneumoniae (DRSP) has also been a global concern, especially penicillin resistance. This study determined the serotypes of S. pneumoniae strains (n = 95) isolated from nasopharyngeal specimens from children admitted to UMMC from 2013 to 2015. In accordance with previous studies, PCR result showed 40% of NT isolates were successfully typed as 3 less common serotypes, namely 9N/L, 17A, and 23B. The repetitive-element PCR (REP-PCR) result revealed genetic variations among the strains whereby five major clusters were observed at the similarity of 80% by clustering analysis based on fingerprint data. Penicillin-binding proteins (pbps) of selected isolates were studied by PCR and sequencing. Three strains with <= 19-mm diameter zone for Oxacillin Disc Diffusion (ODD) test previously were recorded to have mutation on all pbp1a, pbp2b, and pbp2x with MIC of 4 mu g/ml, which were penicillin-intermediate resistance according to the CLSI breakpoints.This publication has 43 references indexed in Scilit:
- Serotypes and Patterns of Antibiotic Resistance in Strains Causing Invasive Pneumococcal Disease in Children Less than 5 Years of AgePLOS ONE, 2013
- Serotyping of Streptococcus pneumoniae Isolates from Nasopharyngeal Samples: Use of an Algorithm Combining Microbiologic, Serologic, and Sequential Multiplex PCR TechniquesJournal of Clinical Microbiology, 2011
- Capsular Serotype and Antibiotic Resistance of Streptococcus pneumoniae Isolates in MalaysiaPLOS ONE, 2011
- Clonal Diversity of Nosocomial Epidemic Acinetobacter baumannii Strains Isolated in SpainJournal of Clinical Microbiology, 2011
- Summary of invasive pneumococcal disease burden among children in the Asia-Pacific regionVaccine, 2010
- Progress toward Rapid and Accurate Staphylococcus aureus Strain TypingClinical Chemistry, 2009
- Discovery of a New Capsular Serotype (6C) within Serogroup 6 ofStreptococcus pneumoniaeJournal of Clinical Microbiology, 2007
- Molecular characterisation of Canadian paediatric multidrug-resistant Streptococcus pneumoniae from 1998–2004International Journal of Antimicrobial Agents, 2006
- A molecular-capsular-type prediction system for 90 Streptococcus pneumoniae serotypes using partial cpsA-cpsB sequencing and wzy- or wzx-specific PCRJournal of Medical Microbiology, 2005
- Alterations of Penicillin-Binding Proteins 1A, 2X, and 2B in Streptococcus pneumoniae Isolates for Which Amoxicillin MICs Are Higher than Penicillin MICsAntimicrobial Agents and Chemotherapy, 2004