Identification and characterization of compounds from Chrysosporium multifidum, a fungus with moderate antimicrobial activity isolated from Hermetia illucens gut microbiota
Open Access
- 20 December 2019
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 14 (12), e0218837
- https://doi.org/10.1371/journal.pone.0218837
Abstract
The gut microbiota of insects is composed of a wide range of microorganisms which produce bioactive compounds that protect their host from pathogenic attack. In the present study, we isolate and identify the fungus Chrysosporium multifidum from the gut of Hermetia illucens larvae. Extract from C. multifidum culture broth supernatant showed moderate activity against a strain of methicillin-resistant Staphylococcus aureus (MRSA). Bioguided isolation of the extract resulted in the characterization of six α-pyrone derivatives (1–6) and one diketopiperazine (7). Of these compounds, 5,6-dihydro-4-methoxy-6-(1-oxopentyl)-2H-pyran-2-one (4) showed the greatest activity (IC50 = 11.4 ± 0.7 μg/mL and MIC = 62.5 μg/mL) against MRSA.Keywords
Funding Information
- FONDECYT (108-2015-FONDECYT)
This publication has 45 references indexed in Scilit:
- Phytotoxic α-pyrones produced by Pestalotiopsis guepinii, the causal agent of hazelnut twig blightThe Journal of Antibiotics, 2012
- Caryophyllenes from a Fungal Culture of Chrysosporium pilosumJournal of Natural Products, 2009
- Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substancesNature Protocols, 2008
- Ultrastructure of the Mesonotal Mycangium of Xylosandrus mutilatus (Blandford), an Exotic Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae) by Light, Scanning, and Transmission Electron MicroscopyMicroscopy and Microanalysis, 2005
- ChemInform Abstract: 14-(2′,3′,5′-Trihydroxyphenyl)tetradecan-2-ol, a Novel Acetylcholinesterase Inhibitor from Chrysosporium sp.ChemInform, 2002
- A New Reaction of PatulinJournal of Natural Products, 1989
- Studies on the bacterial gall of Myrica rubra. Isolation of a new (7,0)-metacyclophan from the gall and DL-.BETA.-phenyllactic acid from the culture of gall-forming bacteria.CHEMICAL & PHARMACEUTICAL BULLETIN, 1987
- Metabolites of an Unidentified Fungus: a New 5,6-Dihydro-2-pyrone Related to PestalotinCanadian Journal of Chemistry, 1974
- Structure of a new fungal lactone, LL-P880.alpha., from an unidentified Penicillium speciesThe Journal of Organic Chemistry, 1972
- Metabolites from a Chrysosporium speciesCanadian Journal of Microbiology, 1971