Boron Supplementation Promotes Osteogenesis of Tibia by Regulating the Bone Morphogenetic Protein-2 Expression in African Ostrich Chicks
- 1 April 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Biological Trace Element Research
- Vol. 199 (4), 1544-1555
- https://doi.org/10.1007/s12011-020-02258-w
Abstract
The present study aimed to explore the effects of supplemental boron on osteogenesis of tibia and to investigate the possible relationship between additional boron and the expression of bone morphogenetic protein-2 (BMP-2) in tibia of ostrich chicks. Therefore, forty-eight African ostrich chicks (15 days old) were supplemented with 0 mg/L, 40 mg/L, 80 mg/L, 160 mg/L, 320 mg/L, and 640 mg/L of boron in drinking water for 75 days. The paraffin sections of tibia used to measure histomorphometric parameters by hematoxylin and eosin (HE) staining, Masson's staining, and immunohistochemistry (IHC). Enzyme-linked immunosorbent assay was performed to assess the level of BMP-2, osteocalcin (BGP), glucocorticoids (GCs), osteoprotegerin (OPG), and receptor activator of nuclear factor kappa-B ligand (RANKL) in serum. TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) technique was performed to detect the cell apoptosis. The results indicated that low dose of supplemental boron (40 mg/L-160 mg/L) in drinking water promotes bone development by increasing the mature ossein. The expression of BMP2 on 45 days was higher than 90 days. Serum level of BMP-2, BGP, and GCs changed significantly in groups with low dosage of boron, and OPG/RANKL ratio was upregulated from 0 to 160 mg/L. Cell apoptosis was least in 40 mg/L and 160 mg/L groups. Taken together, low dose of boron supplemented in drinking water could promote osteogenesis and growth and development of tibia by regulating the expression and secretion of BMP-2 and providing a dynamically balanced environment for tibia growth, development, and reconstruction by regulating the concentrations of BGP, GCs, and OPG/RANKL ratio in serum.Funding Information
- National Natural Science Foundation of China (31672504, 31272517)
This publication has 58 references indexed in Scilit:
- Endocrine Actions of OsteocalcinInternational Journal of Endocrinology, 2013
- A Comparative Analysis of the Osteogenic Effects of BMP-2, FGF-2, and VEGFA in a Calvarial Defect ModelTissue Engineering, Part A, 2012
- BMP3 Suppresses Osteoblast Differentiation of Bone Marrow Stromal Cells via Interaction with Acvr2bMolecular Endocrinology, 2012
- BMP2, but not BMP4, is crucial for chondrocyte proliferation and maturation during endochondral bone developmentJournal of Cell Science, 2011
- Boron regulates mineralized tissue-associated proteins in osteoblasts (MC3T3-E1)Journal of Trace Elements in Medicine and Biology, 2010
- Conditional deletion of BMP7 from the limb skeleton does not affect bone formation or fracture repairJournal of Orthopaedic Research, 2009
- Expression and Synthesis of Bone Morphogenetic Proteins by Osteoclasts: A Possible Path to Anabolic Bone RemodelingJournal of Histochemistry & Cytochemistry, 2008
- Genetic Analysis of the Roles of BMP2, BMP4, and BMP7 in Limb Patterning and SkeletogenesisPLoS Genetics, 2006
- BMP-7 is an inducer of nephrogenesis, and is also required for eye development and skeletal patterning.Genes & Development, 1995
- Dietary boron modified the effects of magnesium and molybdenum on mineral metabolism in the cholecalciferol-deficient chickBiological Trace Element Research, 1989