Evaluation of Pathogen Reduction Systems to Inactivate Dengue and Chikungunya Viruses in Apheresis Platelets Suspended in Plasma
Open Access
- 1 January 2013
- journal article
- research article
- Published by Scientific Research Publishing, Inc. in Advances in Infectious Diseases
- Vol. 03 (01), 1-9
- https://doi.org/10.4236/aid.2013.31001
Abstract
The risk of blood-borne transmission of infectious diseases has led to an increasing awareness of the need for a safe and effective pathogen reduction technology. This study evaluated the efficacy of 2 pathogen reduction systems to inactivate dengue virus (DENV-2) and chikungunya virus (CHIKV) spiked into apheresis platelets (APLT) concentrates. Double-dose APLT collections (n = 3) were split evenly into two units and spiked with 107 infectious units of DENV-2 or CHIKV. APLTs samples were assayed for viral infectivity before and after Amotosalen photochemical treatment (PCT) or Riboflavin pathogen reduction treatment (PRT). Viral infectivity was determined by plaque assays. Platelet (PLT) count, pH and residual S-59 were measured during the storage of 5 days. Amotosalen PCT showed robust efficacy and complete inactivation of both viruses in APLTs, with up to 3.01 and 3.75 log reductions of DENV-2 and CHIKV respectively. At similar initial concentrations, Riboflavin PRT showed complete inactivation of CHIKV with up to 3.73 log reduction, much higher efficacy than against DENV-2 where a log reduction of up to 1.58 was observed. All post-treated APLTs maintained acceptable PLT yields and quality parameters. This parallel study of 2 pathogen reduction systems demonstrates their efficacy in inactivating or reducing DENV and CHIKV in APLTs and reaffirms the usefulness of pathogen inactivation systems to ensure the safety in PLTs transfusion.Keywords
This publication has 30 references indexed in Scilit:
- Photochemical inactivation of chikungunya virus in plasma and platelets using the Mirasol pathogen reduction technology systemTransfusion, 2012
- Dengue viremia in blood donors identified by RNA and detection of dengue transfusion transmission during the 2007 dengue outbreak in Puerto RicoTransfusion, 2012
- Best Practices in Dengue Surveillance: A Report from the Asia-Pacific and Americas Dengue Prevention BoardsPLoS Neglected Tropical Diseases, 2010
- Genetic analysis of chikungunya viruses imported to mainland China in 2008Virology Journal, 2010
- Re-emergence of Chikungunya virus in South-east Asia: virological evidence from Sri Lanka and SingaporeJournal of General Virology, 2009
- Entomologic and Virologic Investigation of Chikungunya, SingaporeEmerging Infectious Diseases, 2009
- Chikungunya Outbreak, Singapore, 2008Emerging Infectious Diseases, 2009
- Is dengue a threat to the blood supply?Transfusion Medicine, 2009
- Threat of Dengue to Blood Safety in Dengue-Endemic CountriesEmerging Infectious Diseases, 2009
- Novel Chikungunya Virus Variant in Travelers Returning from Indian Ocean IslandsEmerging Infectious Diseases, 2006