CRISPR–Cas12-based detection of SARS-CoV-2
Top Cited Papers
- 16 April 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 38 (7), 870-874
- https://doi.org/10.1038/s41587-020-0513-4
Abstract
An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR–Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated our method using contrived reference samples and clinical samples from patients in the United States, including 36 patients with COVID-19 infection and 42 patients with other viral respiratory infections. Our CRISPR-based DETECTR assay provides a visual and faster alternative to the US Centers for Disease Control and Prevention SARS-CoV-2 real-time RT–PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.Keywords
Funding Information
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases (R33-AI129455)
- U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (R01-HL105704)
This publication has 22 references indexed in Scilit:
- A multiplexed RT-LAMP assay for detection of group M HIV-1 in plasma or whole bloodJournal of Virological Methods, 2018
- Field-deployable viral diagnostics using CRISPR-Cas13Science, 2018
- CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activityScience, 2018
- CRISPR-Cas12a-assisted nucleic acid detectionCell Discovery, 2018
- Nucleic acid detection with CRISPR-Cas13a/C2c2Science, 2017
- Data, disease and diplomacy: GISAID's innovative contribution to global healthGlobal Challenges, 2017
- Dehydrogenation of Formic Acid Catalyzed by a Ruthenium Complex with an N,N′-Diimine LigandInorganic Chemistry, 2016
- Analysis Tool Web Services from the EMBL-EBINucleic Acids Research, 2013
- Jalview Version 2—a multiple sequence alignment editor and analysis workbenchBioinformatics, 2009
- Loop-mediated isothermal amplification of DNANucleic Acids Research, 2000