On-Site and Quantitative Detection of Trace Methamphetamine in Urine/Serum Samples with a Surface-Enhanced Raman Scattering-Active Microcavity and Rapid Pretreatment Device

Abstract

Here it reports a high-throughput detection method for reliably quantitative analysis of illegal drugs in complex biological samples by means of surface-enhanced Raman scattering (SERS) active microcavity and rapid pretreatment device. Based on the well-made hemispherical microcavities that regularly distributed on a glass array, the quality controllable microcavity device is fabricated by the compactly self-assembly of core-shell nanopeanuts (CSNPs) onto the inside surface. Both of the CSNPs with quantifiable internal standard signal of crystal violet acetate (CVa) anchored inside their gap and the well-made microcavity referred to the physical amplification of microscale groove surface will do well in trace analysis that allow us to realize the accurately quantitative SERS analysis of targeted analytes spread on the bottom area of microcavity array. As an example, 0.8 nM malachite green (MG) and 160 ppb methamphetamine (MATM) have been successively detected in a wide range as standard, while even 0.01 ppm methamphetamine mixed in the urine/serum samples has been efficiently test-ed by the microcavity device equipped with rapid pretreatment device (manual monolithic column syringe needle). All the above words suggest that the SERS active microcavity equipped with rapid pretreatment device is potential in the on-site quick test of trace amounts of illegal drugs in bodily fluid samples or other field analysis of food sanitation, environmental safety, and public health.