Determination of a novel anticonvulsant drug GIZh-290 in rat plasma by liquid chromatography–tandem mass spectrometry
Open Access
- 17 January 2022
- journal article
- Published by Publishing House OKI in Pharmacokinetics and Pharmacodynamics
- No. 2,p. 3-10
- https://doi.org/10.37489/2587-7836-2021-2-3-10
Abstract
GIZh-290 is a new derivative of 4-phenylpyrrolidone, based on the structures of racetams that combine anticonvulsant and nootropic effects. The method of high-performance liquid chromatography with mass spectrometric detection was developed and validated for the quantitative determination of a new potential anticonvulsant compound GIZh-290 in rat blood plasma. For the extraction of the analyte, liquid-liquid extraction with diethyl ether was used. The method was validated based on its selectivity, linearity, accuracy, precision, and stability. The linearity of the method was confirmed by a high correlation coefficient of 0.999. The percent recovery of GIZh-290 from blood plasma was 79.2 %. The intra- and inter-batch accuracy were 19.32 and 12.14 %. It was found that blood plasma samples containing GIZh-290 can be stored at ambient temperature during the working day.Keywords
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