ABO Genotypes Were Identified by PCR-RFLP Method

Abstract

ABO基因中几个碱基的替换、单碱基缺失导致A、B、O三种等位基因的产生，可使红细胞膜表面带有不同抗原，进而形成四种血型表型。根据三种等位基因关键位点的不同，采用PCR-RFLP方法扩增DNA片段并进行特异性酶切，根据酶切结果可以初步判断基因型，再通过测序的方法验证酶切结果。本实验对笔者和另一名同学进行了ABO基因型鉴定，结果显示，PCR-RFLP结果与测序结果相一致。 The substitution of several bases in ABO gene and the deletion of single base lead to the produc-tion of A, B and O alleles, which can make the erythrocyte membrane surface with different anti-gens, and thus form four blood type phenotypes. According to the differences of the key loci of the three alleles, the PCR-RFLP method was used to amplify the DNA fragments and perform specific enzyme digestion. The genotype could be preliminarily determined based on the enzyme digestion results, and then the enzyme digestion results are verified by sequencing method. In this study, ABO genotypes were identified for the first author and another student, and the results showed that the PCR-RFLP results were consistent with the sequencing results.