Genetic Stability The Protein Encoding Envelope (E) Genes Dengue Virus Serotype-4 Passaged in Vero Cell As A Candidate Chimera Vaccine Material
Open Access
- 23 October 2020
- journal article
- Published by Universitas Airlangga in Journal of Stem Cell Research and Tissue Engineering
- Vol. 4 (2), 40-48
- https://doi.org/10.20473/jscrte.v4i2.22748
Abstract
This study aims to analyze genetic stability of the gene encoding the envelope protein (E) dengue virus serotype-4 passaged in vero cells, Denv-4 passaged in vero cells serially then continued with RNA extraction at passage 0, 10, 20, 30, 40 ,50 , and 60, and then continued with two step PCR and amplification, and sequencing then analyze the nucleotide stability with BLAST and MEGA 5 software. The result shows that there are many variable site in nucleotide and amino acid with high mutation rate 57.4% for nucleotide and 71.9% for amino acid ,while the similarity between passages are high ranging from 91% - 98%. The conclusion for this study is Denv-4 after analyzed shows that the gene encoding protein E has many variable site but high in similarity.Keywords
This publication has 9 references indexed in Scilit:
- Using recombinant DNA technology for the development of live-attenuated dengue vaccinesEnzyme and Microbial Technology, 2012
- The development of recombinant subunit envelope-based vaccines to protect against dengue virus induced diseaseVaccine, 2011
- Genetic stability of a dengue vaccine based on chimeric yellow fever/dengue virusesVaccine, 2011
- Stability of RNA virus attenuation approachesVaccine, 2011
- The Growth Study of Vero Cells in Different Type of MicrocarrierMaterials Sciences and Applications, 2010
- High Genetic Stability of Dengue Virus Propagated in MRC-5 Cells as Compared to the Virus Propagated in Vero CellsPLOS ONE, 2008
- The molecular epidemiology of dengue virus serotype 4 in Bangkok, ThailandVirology, 2004
- Mutations which enhance the replication of dengue virus type 4 and an antigenic chimeric dengue virus type 2/4 vaccine candidate in Vero cellsVaccine, 2003
- Structure of Dengue VirusCell, 2002