Control of pH by CO2 Pressurization for Enzymatic Saccharification of Ca(OH)2-Pretreated Rice Straw in the Presence of CaCO3
Open Access
- 20 May 2020
- journal article
- research article
- Published by The Japanese Society of Applied Glycoscience in Journal of Applied Glycoscience
- Vol. 67 (2), 59-62
- https://doi.org/10.5458/jag.jag.jag-2019_0019
Abstract
The aim of this study was to investigate the effect of pH control by CO2 pressurization on the enzymatic hydrolysis of herbaceous feedstock in the calcium capturing by carbonation (CaCCO) process for fermentable sugar production. The pH of the slurry of 5% (w/w) Ca(OH)2-pretreated/CO2-neutralized rice straw could be controlled between 5.70 and 6.38 at 50 °C by changing the CO2 partial pressure (pCO2) from 0.1 to 1.0 MPa. A mixture of fungal enzyme preparations, namely, Trichoderma reesei cellulases/hemicellulases and Aspergillus niger β-glucosidase, indicated that pH 5.5–6.0 is optimal for solubilizing sugars from Ca(OH)2-pretreated rice straw. Enzymatic saccharification of pretreated rice straw under various pCO2 conditions revealed that the highest soluble sugar yields were obtained at pCO2 0.4 MPa and over, which is consistent with the expected pH at the pCO2 without enzymes and demonstrates the effectiveness of pH control by CO2 pressurization.Keywords
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