LncRNA FAM83A‐AS1 promotes lung adenocarcinoma progression by enhancing the pre‐mRNA stability of FAM83A
Open Access
- 9 March 2021
- journal article
- research article
- Published by Wiley in Thoracic Cancer
- Vol. 12 (10), 1495-1502
- https://doi.org/10.1111/1759-7714.13928
Abstract
Background Lung cancer is the leading cause of cancer deaths worldwide. Long non‐coding RNAs (lncRNAs) affect a series of cellular biological processes, including oncogene function promotion. In this study, we explored the functions and mechanisms of FAM83A antisense RNA 1 (FAM83A‐AS1) in non‐small cell lung cancer (NSCLC) progression. Methods The expression of FAM83A‐AS1and FAM83A mRNA was analyzed using the Cancer Genome Atlas (TCGA) data. Proliferation, migration, invasion and Western blotting were measured after treatment with overexpressed or knockdown FAM83A‐AS1. To determine the relationship between FAM83A‐AS1 and FAM83A, RNase protection assay (RPA), amanitin treatment, RNA pulldown assay and RNA immunoprecipitation (RIP) assay were performed. Results High expression of FAM83A‐AS1 in lung adenocarcinoma (LUAD) was closely associated with low overall survival (OS) and progression‐free survival (PFS). Functionally, high FAM83A‐AS1 expression increased LUAD cell proliferation and metastasis, indicating that FAM83A‐AS1 exerted its oncogenic functions. Furthermore, FAM83A‐AS1 promoted NSCLC progression via ERK signaling pathways. Mechanistically, FAM83A‐AS1 post‐transcriptionally increased FAM83A expression by enhancing pre‐mRNA stability. FAM83A‐AS1 enhanced FAM83A mRNA stability not only by forming an RNA duplex but also by binding to FBL. Conclusions We determined that FAM83A‐AS1 increased FAM83A expression by enhancing FAM83A pre‐mRNA stability and promoted the tumorigenesis of LUAD.Keywords
Funding Information
- National Natural Science Foundation of China (81672934, 81873417)
This publication has 32 references indexed in Scilit:
- Long Noncoding RNAs in Cancer PathwaysCancer Cell, 2016
- A novel cell cycle-associated lncRNA, HOXA11-AS, is transcribed from the 5-prime end of the HOXA transcript and is a biomarker of progression in gliomaCancer Letters, 2016
- Cancer statistics, 2016CA: A Cancer Journal for Clinicians, 2016
- RNA binding protein RBM3 increases β‐catenin signaling to increase stem cell characteristics in colorectal cancer cellsMolecular Carcinogenesis, 2015
- The landscape of antisense gene expression in human cancersGenome Research, 2015
- The Landscape of long noncoding RNA classificationTrends in Genetics, 2015
- Neighboring Gene Regulation by Antisense Long Non-Coding RNAsInternational Journal of Molecular Sciences, 2015
- The evolutionarily conserved multifunctional glycine‐rich RNA‐binding proteins play key roles in development and stress adaptationPhysiologia Plantarum, 2014
- Bidirectional regulation between WDR83 and its natural antisense transcript DHPS in gastric cancerCell Research, 2012
- Expression of a noncoding RNA is elevated in Alzheimer's disease and drives rapid feed-forward regulation of β-secretaseNature Medicine, 2008