In Situ Surface Modification of Microfluidic Blood–Brain-Barriers for Improved Screening of Small Molecules and Nanoparticles
- 23 November 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Applied Materials & Interfaces
- Vol. 12 (51), 56753-56766
- https://doi.org/10.1021/acsami.0c17102
Abstract
Here, we have developed and evaluated a microfluidic-based human blood–brain-barrier (μBBB) platform that models and predicts brain tissue uptake of small molecule drugs and nanoparticles (NPs) targeting the central nervous system. By using a photocrosslinkable copolymer that was prepared from monomers containing benzophenone and N-hydroxysuccinimide ester functional groups, we were able to evenly coat and functionalize μBBB chip channels in situ, providing a covalently attached homogenous layer of extracellular matrix proteins. This novel approach allowed the coculture of human endothelial cells, pericytes, and astrocytes and resulted in the formation of a mimic of cerebral endothelium expressing tight junction markers and efflux proteins, resembling the native BBB. The permeability coefficients of a number of compounds, including caffeine, nitrofurantoin, dextran, sucrose, glucose, and alanine, were measured on our μBBB platform and were found to agree with reported values. In addition, we successfully visualized the receptor-mediated uptake and transcytosis of transferrin-functionalized NPs. The BBB-penetrating NPs were able to target glioma cells cultured in 3D in the brain compartment of our μBBB. In conclusion, our μBBB was able to accurately predict the BBB permeability of both small molecule pharmaceuticals and nanovectors and allowed time-resolved visualization of transcytosis. Our versatile chip design accommodates different brain disease models and is expected to be exploited in further BBB studies, aiming at replacing animal experiments.Keywords
Funding Information
- Science and Industry Endowment Fund (PF16-072)
- Australian Research Council (IC170100016)
- Commonwealth Scientific and Industrial Research Organisation
- National Natural Science Foundation of China (22004099)
- Northwestern Polytechnical University (2020GXLH-Z-008, 2020GXLH-Z-023)
- Foundation for Children (EOI. 2016-081)
- Department of Science and Technology of Shaanxi Province
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