The epidemiology of animal rotaviruses infection and their association with illnesses in humans remains unknown in Central America and the world. In this study, we performed a genotyping of rotavirus strains by RT-PCR for-G (VP7) and P (VP4), in samples of diarrhea in calves and piglets, in Honduras and Panama. Mixed infections and combinations of genotypes G (VP7) and P (VP4) were identified in calves in Honduras: 3 (4%) G2P [6], 2 (2%) G2P [4], in piglets: 4 (5%) G4P [6], and in calves in Panama: 63 (72%) G10 P [2], 12 (13%) G8 P [11], 9 (10%) G6 P [11] and 3 (3%) G10 P [1].This investigation represents the first report of bovine and porcine rotavirus strains in Honduras and Panama.