Occurrence of Listeria spp. and Listeria monocytogenes Isolated from PDO Taleggio Production Plants
Open Access
- 9 November 2020
- Vol. 9 (11), 1636
- https://doi.org/10.3390/foods9111636
Abstract
The present study evaluated the presence of Listeria spp. and L. monocytogenes in four plants producing PDO Taleggio cheese. A total of 360 environmental samples were collected from different areas during production. The sampling points were identified as Food Contact Surfaces (FCS), transfer-Non Food Contact Surfaces (tr-NFCS), and non-transfer-NFCS (non-tr-NFCS). Fifty-nine ingredients/products were also analyzed. Listeria spp. was found in all the plants with a mean prevalence of 23.1%; plants that included a ripening area showed significantly higher prevalence if compared to the other plants. The positivity rate detected on FCS was moderate (~12%), but significantly lower if compared to NFCS (about 1/4 of the samples, p < 0.01). Among the FCS, higher prevalence was revealed on ripening equipment. Listeria spp. was never detected in the ingredients or products. A total of 125 Listeria spp. isolates were identified, mostly as L. innocua (almost 80%). L. monocytogenes was detected only from two FCS samples, in an area dedicated to the cutting of ripened blue cheeses; strain characterization by whole genome sequencing (WGS) evidenced a low virulence of the isolates. The results of the present study stress the importance of Listeria spp. management in the dairy plants producing PDO Taleggio and similar cheeses, mainly by the application of strict hygienic practices.This publication has 33 references indexed in Scilit:
- Increased In Vitro Adherence and On-Farm Persistence of Predominant and Persistent Listeria monocytogenes Strains in the Milking SystemApplied and Environmental Microbiology, 2011
- Listeria monocytogenes in Irish Farmhouse cheese processing environmentsInternational Journal of Food Microbiology, 2011
- Large Listeriosis Outbreak Linked to Cheese Made from Pasteurized Milk, Germany, 2006–2007Foodborne Pathogens & Disease, 2010
- Measuring dementia carers' unmet need for services - an exploratory mixed method studyBMC Health Services Research, 2010
- Evaluation of a multiplex PCR assay as an alternative method for Listeria monocytogenes serotypingJournal of Microbiological Methods, 2010
- Molecular Ecology of Listeria monocytogenes : Evidence for a Reservoir in Milking Equipment on a Dairy FarmApplied and Environmental Microbiology, 2009
- Microbiological quality of retail cheeses made from raw, thermized or pasteurized milk in the UKFood Microbiology, 2008
- Occurrence and ribotypes of Listeria monocytogenes in Gorgonzola cheesesInternational Journal of Food Microbiology, 2005
- Differentiation of the Major Listeria monocytogenes Serovars by Multiplex PCRJournal of Clinical Microbiology, 2004
- Fatal Case of Listeria innocua BacteremiaJournal of Clinical Microbiology, 2003