Rice phenolamindes reduce the survival of female adults of the white-backed planthopper Sogatella furcifera

Abstract

In response to infestation by herbivores, rice plants rapidly biosynthesize defense compounds by activating a series of defense-related pathways. However, which defensive compounds in rice are effective against herbivores remains largely unknown. We found that the infestation of white-backed planthopper (WBPH) Sogatella furcifera gravid females significantly increased levels of jasmonic acid (JA), jasmonoyl-isoleucine (JA-Ile) and H₂O₂, and reduced the level of ethylene in rice; levels of 11 of the tested 12 phenolamides (PAs) were subsequently enhanced. In contrast, WBPH nymph infestation had no effect on levels of JA, JA-Ile, ethylene and H₂O₂ in rice, and enhanced levels of only 2 of 12 PAs. Moreover, infestation by brown planthopper Nilaparvata lugens gravid females also affected the production of these PAs differently. Bioassays revealed that 4 PAs – N-feruloylputrescine, N-feruloyltyramine, feruloylagmatine and N1,N10-diferuloylspermidine – were toxic to newly emerged WBPH female adults. Our results suggest that WBPH- or BPH-induced biosynthesis of PAs in rice seems to be shaped primarily by the specific profile of defense-related signals elicited by the herbivore and that PAs play a role in conferring the resistance to WBPH on rice.