mRNAs, proteins and the emerging principles of gene expression control
Top Cited Papers
- 1 October 2020
- journal article
- review article
- Published by Springer Science and Business Media LLC in Nature Reviews Genetics
- Vol. 21 (10), 630-644
- https://doi.org/10.1038/s41576-020-0258-4
Abstract
Gene expression is typically measured at the level of either mRNAs or proteins. In this Review, Buccitelli and Selbach discuss how large-scale comparative studies are characterizing the degree to which mRNA and protein levels correlate. They discuss technical and biological reasons why mRNA and protein levels may be particularly concordant or disconnected, as well as the different biological information provided by these non-redundant readouts of gene expression. Gene expression involves transcription, translation and the turnover of mRNAs and proteins. The degree to which protein abundances scale with mRNA levels and the implications in cases where this dependency breaks down remain an intensely debated topic. Here we review recent mRNA-protein correlation studies in the light of the quantitative parameters of the gene expression pathway, contextual confounders and buffering mechanisms. Although protein and mRNA levels typically show reasonable correlation, we describe how transcriptomics and proteomics provide useful non-redundant readouts. Integrating both types of data can reveal exciting biology and is an essential step in refining our understanding of the principles of gene expression control.This publication has 178 references indexed in Scilit:
- Protein synthesis rate is the predominant regulator of protein expression during differentiationMolecular Systems Biology, 2013
- Quantitative Analysis of Fission Yeast Transcriptomes and Proteomes in Proliferating and Quiescent CellsCell, 2012
- Global analysis of genome, transcriptome and proteome reveals the response to aneuploidy in human cellsMolecular Systems Biology, 2012
- Ribosome Profiling of Mouse Embryonic Stem Cells Reveals the Complexity and Dynamics of Mammalian ProteomesCell, 2011
- Global quantification of mammalian gene expression controlNature, 2011
- Ubiquitination of E3 ligases: self-regulation of the ubiquitin system via proteolytic and non-proteolytic mechanismsCell Death & Differentiation, 2011
- Identification of Aneuploidy-Selective Antiproliferation CompoundsCell, 2011
- Emerging properties of animal gene regulatory networksNature, 2010
- A signature of chromosomal instability inferred from gene expression profiles predicts clinical outcome in multiple human cancersNature Genetics, 2006
- Mass spectrometry‐based metabolomicsMass Spectrometry Reviews, 2006