Unique Cell Surface Mannan of Yeast Pathogen Candida auris with Selective Binding to IgG
- 8 May 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Infectious Diseases
- Vol. 6 (5), 1018-1031
- https://doi.org/10.1021/acsinfecdis.9b00450
Abstract
The emerging, multidrug-resistant yeast pathogen Candida auris is responsible for healthcare-associated outbreaks across the globe with high mortality. The rapid spread of C. auris is linked to its successful colonization of human skin, followed by bloodstream infections. We compared glycomics and proteomics of C. auris to closely and distantly related human pathogenic yeasts, C. haemulonii and C. albicans, with the aim to understand the role of cell surface molecules in skin colonization and immune system interactions. Candida auris mannan is distinct from other pathogenic Candida species, as it is highly enriched in beta-1,2-linkages. The experimental data showed that C. auris surface mannan beta-1,2-linkages were important for the interactions with the immune protein IgG, found in blood and in sweat glands, and with the mannose binding lectin, found in the blood. Candida auris mannan binding to IgG was from 12- to 20-fold stronger than mannan from the more common pathogen C. albicans. The findings suggest unique C. auris mannan could be crucial for the biology and pathogenesis of this emerging pathogen.Funding Information
- U.S. Department of Health and Human Services (CA231074, DK111958)
- U.S. Department of Health and Human Services (DK111958)
- New York State Department of Health - Wadsworth Center
This publication has 44 references indexed in Scilit:
- Structural basis for promiscuity and specificity during Candida glabrata invasion of host epitheliaProceedings of the National Academy of Sciences of the United States of America, 2012
- A β-mannan trisaccharide conjugate vaccine aids clearance of Candida albicans in immunocompromised rabbitsVaccine, 2012
- Identification of Candida albicans exposed surface proteins in vivo by a rapid proteomic approachJournal of Proteomics, 2010
- Candida aurissp. nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a Japanese hospitalMicrobiology and Immunology, 2009
- Synthetic glycopeptide vaccines combining β-mannan and peptide epitopes induce protection against candidiasisProceedings of the National Academy of Sciences of the United States of America, 2008
- Dendritic Cell Interaction with Candida albicans Critically Depends on N-Linked MannanOnline Journal of Public Health Informatics, 2008
- Chemical structure of the cell-wall mannan of Candida albicans serotype A and its difference in yeast and hyphal formsBiochemical Journal, 2007
- Existence of Branched Side Chains in the Cell Wall Mannan of Pathogenic Yeast, Candida albicansOnline Journal of Public Health Informatics, 1995
- Sequential nuclear magnetic resonance assignment of .beta.-1,2-linked mannooligosaccharides isolated from the phosphomannan of the pathogenic yeast Candida albicans NIH B-792 strainBiochemistry, 1992
- Mannan antigenemia in the diagnosis of invasive Candida infections.JCI Insight, 1976