Differences in grapevine rootstock sensitivity and recovery from drought are linked to fine root cortical lacunae and root tip function
- 1 January 2021
- journal article
- research article
- Published by Wiley in New Phytologist
- Vol. 229 (1), 272-283
- https://doi.org/10.1111/nph.16542
Abstract
Structural changes during severe drought stress greatly modify the hydraulic properties of fine roots. Yet, the physiological basis behind the restoration of fine root water uptake capacity during water recovery remains unknown. Using neutron radiography (NR), X-ray micro-computed tomography (micro-CT), fluorescence microscopy, and fine root hydraulic conductivity measurements (Lp(r)), we examined how drought-induced changes in anatomy and hydraulic properties of contrasting grapevine rootstocks are coupled with fine root growth dynamics during drought and return of soil moisture. Lacunae formation in drought-stressed fine roots was associated with a significant decrease in fine root Lp(r) for both rootstocks. However, lacunae formation occurred under milder stress in the drought-resistant rootstock, 110R. Suberin was deposited at an earlier developmental stage in fine roots of 101-14Mgt (i.e. drought susceptible), probably limiting cortical lacunae formation during mild stress. During recovery, we found that only 110R fine roots showed rapid re-establishment of elongation and water uptake capacity and we found that soil water status surrounding root tips differed between rootstocks as imaged with NR. These data suggest that drought resistance in grapevine rootstocks is associated with rapid re-establishment of growth and Lp(r) near the root tip upon re-watering by limiting competing sites along the root cylinder.Keywords
Funding Information
- Fondo Nacional de Desarrollo Científico y Tecnológico (11180102)
This publication has 59 references indexed in Scilit:
- Water Uptake along the Length of Grapevine Fine Roots: Developmental Anatomy, Tissue-Specific Aquaporin Expression, and Pathways of Water TransportPlant Physiology, 2013
- Root traits contributing to plant productivity under droughtFrontiers in Plant Science, 2013
- The relationship between root hydraulics and scion vigour across Vitis rootstocks: what role do root aquaporins play?Journal of Experimental Botany, 2012
- Fiji: an open-source platform for biological-image analysisNature Methods, 2012
- Casparian strip diffusion barrier in Arabidopsis is made of a lignin polymer without suberinProceedings of the National Academy of Sciences, 2012
- Root-Specific Reduction of Cytokinin Causes Enhanced Root Growth, Drought Tolerance, and Leaf Mineral Enrichment in Arabidopsis and TobaccoPlant Cell, 2010
- The Dynamics of Embolism Repair in Xylem: In Vivo Visualizations Using High-Resolution Computed TomographyPlant Physiology, 2010
- The Role of Plasma Membrane Intrinsic Protein Aquaporins in Water Transport through Roots: Diurnal and Drought Stress Responses Reveal Different Strategies between Isohydric and Anisohydric Cultivars of GrapevinePlant Physiology, 2008
- Plant Aquaporins: Membrane Channels with Multiple Integrated FunctionsAnnual Review of Plant Biology, 2008
- Inherent Variation in Growth Rate Between Higher Plants: A Search for Physiological Causes and Ecological ConsequencesPublished by Elsevier BV ,1992