Electrochemiluminescence immunosensor for cytokeratin fragment antigen 21-1 detection using electrochemically mediated atom transfer radical polymerization
Open Access
- 8 March 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Microchimica Acta
- Vol. 188 (4), 1-8
- https://doi.org/10.1007/s00604-020-04677-x
Abstract
The cytokeratin fragment antigen 21-1 (CYFRA 21-1) protein is a critical tumor biomarker tightly related to non-small cell lung cancer (NSCLC). Herein, we prepared an effective electrochemiluminescence (ECL) immunosensor for CYFRA 21-1 detection using electrochemically mediated atom transfer radical polymerization (eATRP). The CYFRA 21-1 antigen was fixed on the electrode surface by constructing a sandwich type antibody-antigen-antibody immune system. The sensitivity of ECL was improved by using the eATRP reaction. In this method, eATRP was applied to CYFRA 21-1 detection antibody with N-acryloyloxysuccinimide as functional monomer. This is the first time that ECL and eATRP signal amplification technology had been combined. Under the optimized testing conditions, the immunosensor showed a good linear relation in the range from 1 fg mL−1 to 1 μg mL−1 at a limit of detection of 0.8 fg mL−1 (equivalent to ~ 134 molecules in a 10 μL sample). The ECL immunosensing system based on eATRP signal amplification technology provided a new way for rapid diagnosis of lung cancer by detecting CYFRA 21-1. Graphical abstractFunding Information
- Project of National Key Research and Development Project (2017YFC1702800)
- National Key Research and Development Project (2019YFC1708802)
- Zhongyuan Leading Talent (ZYQR201810080)
This publication has 31 references indexed in Scilit:
- Aptamer-based biosensor for detecting carcinoembryonic antigenTalanta, 2020
- RETRACTED ARTICLE: Knockdown of hsa_circ_0058124 inhibits the proliferation of human lung cancer cells by up-regulation of miR-1297Artificial Cells, Nanomedicine, and Biotechnology, 2020
- Electrochemical detection of the human cancer biomarker ‘autocrine motility factor-phosphoglucose isomerase’ based on a biosensor formed with a monosaccharidic inhibitorSensors and Actuators B: Chemical, 2019
- Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISABMC Chemistry, 2019
- Ultrasensitive chemiluminescence assay for the lung cancer biomarker cytokeratin 21-1 via a dual amplification scheme based on the use of encoded gold nanoparticles and a toehold-mediated strand displacement reactionMicrochimica Acta, 2017
- Combination of serum miRNAs with Cyfra21-1 for the diagnosis of non-small cell lung cancerCancer Letters, 2015
- Highly sensitive immunoassay of carcinoembryonic antigen by capillary electrophoresis with gold nanoparticles amplified chemiluminescence detectionJournal of Chromatography A, 2013
- A chemiluminescent metalloimmunoassay based on copper-enhanced gold nanoparticles for quantification of human growth hormoneLuminescence, 2012
- Recent advances in electrochemiluminescent enzyme biosensorsTrAC Trends in Analytical Chemistry, 2011
- Development and analytical validation of a radioimmunoassay for the measurement of alpha1-proteinase inhibitor concentrations in feces from healthy puppies and adult dogsJournal of Veterinary Diagnostic Investigation, 2011