Cryogenic 3D printing of porous scaffolds for in situ delivery of 2D black phosphorus nanosheets, doxorubicin hydrochloride and osteogenic peptide for treating tumor resection-induced bone defects
- 30 June 2020
- journal article
- research article
- Published by IOP Publishing in Biofabrication
- Vol. 12 (3), 035004
- https://doi.org/10.1088/1758-5090/ab6d35
Abstract
Tumor resection is widely used to prevent tumor growth. However, the defected tissue at the original tumor site also causes tissue or organ dysfunction which lowers the patient's life quality. Therefore, regenerating the tissue and preventing tumor recurrence are highly important. Herein, according to the concept of 'first kill and then regenerate', a versatile scaffold-based tissue engineering strategy based on cryogenic 3D printing of water-in-oil polyester emulsion inks, containing multiple functional agents, was developed, in order to realize the elimination of tumor cells with recurrence suppression and improved tissue regeneration sequentially. To illustrate our strategy, water/poly (lactic-co-glycolic acid)/dichloromethane emulsions containing beta-tricalcium phosphate (beta-TCP), 2D black phosphorus (BP) nanosheets, low-dose doxorubicin hydrochloride (DOX) and high-dose osteogenic peptide were cryogenically 3D printed into hierarchically porous and mechanically strong nanocomposite scaffolds, with multiple functions to treat bone tumor, resection-induced tissue defects. Prompt tumor ablation and long-term suppression of tumor recurrence could be achieved due to the synergistic effects of photothermotherapy and chemotherapy, and improved bone regeneration was obtained eventually due to the presence of bony environment and sustained peptide release. Notably, BP nanosheets in scaffolds significantly reduced the long-term toxicity phenomenon of releasedDOXduring in vivo bone regeneration. Our study also provides insights for the design of multi-functional tissue engineering scaffolds for treating other tumor resection-induced tissue defects.Funding Information
- Department of Education of Guangdong in China (2017KZDXM082)
- Dongguan University of Technology Research Team (TDYB2019003)
- Dongguan University of Technology high-level talents (innovation team) research project (KCYCXPT201603)
- Natural Science Foundation of Guangdong Province, China (2018A0303130019, 2015A030310313)
- Young Innovative Talent Project from the Department of Education of Guangdong Province, China (2016KQNCX168)
- Natural Science Foundation of China (81772315, 81772428)
This publication has 42 references indexed in Scilit:
- Localized Co-delivery of Doxorubicin, Cisplatin, and Methotrexate by Thermosensitive Hydrogels for Enhanced Osteosarcoma TreatmentACS Applied Materials & Interfaces, 2015
- Repair of rat cranial bone defect by using bone morphogenetic protein-2-related peptide combined with microspheres composed of polylactic acid/polyglycolic acid copolymer and chitosanBiomedical Materials, 2015
- Thermally triggered release of a pro-osteogenic peptide from a functionalized collagen-based scaffold using thermosensitive liposomesJournal of Controlled Release, 2014
- ECM Inspired Coating of Embroidered 3D Scaffolds Enhances Calvaria Bone RegenerationBioMed Research International, 2014
- Lipid–Peptide Vesicle Nanoscale Hybrids for Triggered Drug Release by Mild Hyperthermia in Vitro and in VivoACS Nano, 2012
- The effect of the local delivery of alendronate on human adipose-derived stem cell-based bone regenerationBiomaterials, 2010
- OsteosarcomaAnnals of Oncology, 2010
- In vivo and in vitro evaluation of flexible, cottonwool-like nanocomposites as bone substitute material for complex defectsActa Biomaterialia, 2009
- Drug Resistance and the Solid Tumor MicroenvironmentJNCI Journal of the National Cancer Institute, 2007
- Evaluation of bone regeneration at critical-sized calvarial defect by DBM/AM compositeJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2007