White pupae phenotype of tephritids is caused by parallel mutations of a MFS transporter
Open Access
- 21 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 12 (1), 1-12
- https://doi.org/10.1038/s41467-020-20680-5
Abstract
Mass releases of sterilized male insects, in the frame of sterile insect technique programs, have helped suppress insect pest populations since the 1950s. In the major horticultural pests Bactrocera dorsalis, Ceratitis capitata, and Zeugodacus cucurbitae, a key phenotype white pupae (wp) has been used for decades to selectively remove females before releases, yet the gene responsible remained unknown. Here, we use classical and modern genetic approaches to identify and functionally characterize causal wp− mutations in these distantly related fruit fly species. We find that the wp phenotype is produced by parallel mutations in a single, conserved gene. CRISPR/Cas9-mediated knockout of the wp gene leads to the rapid generation of white pupae strains in C. capitata and B. tryoni. The conserved phenotype and independent nature of wp− mutations suggest this technique can provide a generic approach to produce sexing strains in other major medical and agricultural insect pests.This publication has 70 references indexed in Scilit:
- Transgenic sexing system for Ceratitis capitata (Diptera: Tephritidae) based on female-specific embryonic lethalityInsect Biochemistry and Molecular Biology, 2013
- STAR: ultrafast universal RNA-seq alignerBioinformatics, 2012
- HaploMerger: Reconstructing allelic relationships for polymorphic diploid genome assembliesGenome Research, 2012
- Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence dataBioinformatics, 2012
- A statistical framework for SNP calling, mutation discovery, association mapping and population genetical parameter estimation from sequencing dataBioinformatics, 2011
- HMMER web server: interactive sequence similarity searchingNucleic Acids Research, 2011
- Integrative genomics viewerNature Biotechnology, 2011
- Identification of Functional Elements and Regulatory Circuits by Drosophila modENCODEScience, 2010
- Fine-scale genetic mapping of a hybrid sterility factor between Drosophila simulans and D. mauritiana: The varied and elusive functions of "speciation genes".BMC Evolutionary Biology, 2010
- The Sequence Alignment/Map format and SAMtoolsBioinformatics, 2009