Influence of Simulated Pre-Hospital Transport, Time to Analysis, and Storage Temperature on S100 Calcium-Binding Protein B Values
- 1 September 2020
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Neurotrauma
- Vol. 37 (17), 1864-1869
- https://doi.org/10.1089/neu.2019.6907
Abstract
According to in-hospital guidelines, the biomarker, S100 calcium-binding protein B (S100B), is used to rule out intracranial lesions in mild-moderate traumatic brain injury (TBI). It is currently investigated whether S100B is applicable in a pre-hospital setting. The aim was to compare S100B values and hemolysis index in blood samples drawn and stored under simulated pre-hospital conditions to standardized blood samples. Thirty patients undergoing craniotomy at Department of Neurosurgery, Aarhus University Hospital (Aarhus, Denmark) each had six blood samples drawn. Two samples, drawn in in-hospital standardized Beckton Dickinson tubes and pre-hospital Monovette tubes, respectively, were stored as references at 21 degrees C for 30 min. Two samples were stored at 15 degrees C and 29 degrees C, respectively, one sample was stored at prolonged time (60 min), and one sample was transported for 30 min before centrifugation. S100B values were compared by equivalence test with a pre-defined equivalence margin of +/- 8.5%. There was no clinically relevant difference between samples stored in different tubes, at various temperatures, or time to analysis compared to reference samples. Transported samples had an 11.5% (90% confidence interval [CI], 6.55; 16.61) higher median S100B value and a 430% (95% CI, 279.6; 661.4) higher median hemolysis index compared to reference samples. Three of 30 (10%) patients had an S100B value above guideline cutoff in the transported sample, which was not found in reference samples (false positive). There were no false negatives. In conclusion, S100B values were not influenced by different tubes, temperatures, and time to analysis. Transported samples had higher median S100B values and hemolysis, icterus, and lipemia index compared to reference samples.This publication has 11 references indexed in Scilit:
- Prospective Validation of the Scandinavian Guidelines for Initial Management of Minimal, Mild, and Moderate Head Injuries in AdultsJournal of Neurotrauma, 2019
- Utility and effectiveness of the Scandinavian guidelines to exclude computerized tomography scanning in mild traumatic brain injury - a prospective cohort studyBMC Emergency Medicine, 2018
- Modeling the Kinetics of Serum Glial Fibrillary Acidic Protein, Ubiquitin Carboxyl-Terminal Hydrolase-L1, and S100B Concentrations in Patients with Traumatic Brain InjuryJournal of Neurotrauma, 2017
- A review of the clinical utility of serum S100B protein levels in the assessment of traumatic brain injuryActa Neurochirurgica, 2016
- Kinetic modelling of serum S100b after traumatic brain injuryBMC Neurology, 2016
- Validation of the Scandinavian guidelines for initial management of minimal, mild and moderate traumatic brain injury in adultsBMC Medicine, 2015
- Epidemiology of traumatic brain injury in EuropeActa Neurochirurgica, 2015
- Dynamics of S100B Release into Serum and Cerebrospinal Fluid Following Acute Brain InjuryActa neurochirurgica. Supplement, 2009
- Peri-Operative Levels of S-100 Protein in Serum: Marker for Surgical Manipulation and Postoperative Complicationsmin - Minimally Invasive Neurosurgery, 2003
- Measurements of Serum S-100B Protein: Effects of Storage Time and Temperature on Pre-Analytical Stabilitycclm, 2003