Normal gut microbiome in NMDA receptor encephalitis
Open Access
- 1 November 2019
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Neurology Neuroimmunology & Neuroinflammation
Abstract
Objective To determine whether the gut microbiota shows overabundance of commensal bacteria species in patients with anti-NMDA receptor (NMDAR) encephalitis, similar to patients with MS or neuromyelitis optica where they potentially balance pro- and anti-inflammatory immune responses or participate in disease pathogenesis by molecular mimicry. Methods Intestinal microbiota was characterized in patients with NMDAR encephalitis (n = 23, mean age: 34 ± 12.7 years; 21 females) and age/sex/environment-matched healthy controls (n = 24, 40 ± 14.2 years; 22 females) using stool bacteria 16S rDNA sequencing and classification in operational taxonomic units (OTUs). Statistical analyses focused on intraindividual and interindividual bacterial diversity and identification of differentially abundant taxa. Results Patients with NMDAR encephalitis and controls had similar microbiome profiles of the gut microbiota regarding intraindividual bacterial diversity, OTU distribution, ratio between regional and local species diversity when testing all OTUs, and genera with a relative abundance greater than 0.5%. Similarly, the subgroup of NMDAR encephalitis patients with an ovarian teratoma (n = 3) showed no differences in microbiome variation compared with controls. Patients in the acute encephalitis stage (n = 8) showed significant differences in the numbers of Clostridium XVIII, Clostridium IV, Oscillibacter, Prevotella, and Blautia; however, significance was lost after correction for multiple testing. Conclusion Patients with NMDAR encephalitis and controls both had a normal gut microbiome. The lack of overabundance of certain bacterial species in patients suggests that microbiome changes are no major contributors to the pathogenesis, disease course, or prognosis in NMDAR encephalitis. Despite the small sample size and heterogeneous groups, findings indicate differences to other neuroimmunologic diseases.Keywords
This publication has 26 references indexed in Scilit:
- Guillain–Barré syndrome, transverse myelitis and infectious diseasesCellular & Molecular Immunology, 2018
- The Role of the Gut Microbiome in Multiple Sclerosis Risk and Progression: Towards Characterization of the “MS Microbiome”Neurotherapeutics, 2017
- Gut microbiota from multiple sclerosis patients enables spontaneous autoimmune encephalomyelitis in miceProceedings of the National Academy of Sciences of the United States of America, 2017
- The Human Intestinal Microbiome in Health and DiseaseThe New England Journal of Medicine, 2016
- Gut microbiome analysis in neuromyelitis optica reveals overabundance of Clostridium perfringensAnnals of Neurology, 2016
- Dysbiosis in the Gut Microbiota of Patients with Multiple Sclerosis, with a Striking Depletion of Species Belonging to Clostridia XIVa and IV ClustersPLOS ONE, 2015
- Guillain–Barré syndrome: pathogenesis, diagnosis, treatment and prognosisNature Reviews Neurology, 2014
- Commensal microbiota and myelin autoantigen cooperate to trigger autoimmune demyelinationNature, 2011
- Central Nervous System Demyelinating Disease Protection by the Human Commensal Bacteroides fragilis Depends on Polysaccharide A ExpressionThe Journal of Immunology, 2010
- Proinflammatory T-cell responses to gut microbiota promote experimental autoimmune encephalomyelitisProceedings of the National Academy of Sciences of the United States of America, 2010