Involvement of the MxtR/ErdR (CrbS/CrbR) Two-Component System in Acetate Metabolism in Pseudomonas putida KT2440
Open Access
- 22 July 2021
- journal article
- research article
- Published by MDPI AG in Microorganisms
- Vol. 9 (8), 1558
- https://doi.org/10.3390/microorganisms9081558
Abstract
MxtR/ErdR (also called CrbS/CrbR) is a two-component system previously identified as important for the utilization of acetate in Vibrio cholerae and some Pseudomonas species. In addition, evidence has been found in Pseudomonas aeruginosa for a role in regulating the synthesis and expression, respectively, of virulence factors such as siderophores and RND transporters. In this context, we investigated the physiological role of the MxtR/ErdR system in the soil bacterium Pseudomonas putida KT2440. To that end, mxtR and erdR were individually deleted and the ability of the resulting mutants to metabolize different carbon sources was analyzed in comparison to wild type. We also assessed the impact of the deletions on siderophore production, expression of mexEF-oprN (RND transporter), and the biocontrol properties of the strain. Furthermore, the MxtR/ErdR-dependent expression of putative target genes and binding of ErdR to respective promoter regions were analyzed. Our results indicated that the MxtR/ErdR system is active and essential for acetate utilization in P. putida KT2440. Expression of scpC, pp_0354, and acsA-I was stimulated by acetate, while direct interactions of ErdR with the promoter regions of the genes scpC, pp_0354, and actP-I were demonstrated by an electromobility shift assay. Finally, our results suggested that MxtR/ErdR is neither involved in regulating siderophore production nor the expression of mexEF-oprN in P. putida KT2440 under the conditions tested.Keywords
Funding Information
- Deutsche Forschungsgemeinschaft (JU333/6-1)
- LMUexcellent Postdoc Support Fund ((Ludwig-Maximilians Universität München))
This publication has 34 references indexed in Scilit:
- Transport and kinase activities of CbrA of Pseudomonas putida KT2440Scientific Reports, 2020
- MiST 3.0: an updated microbial signal transduction database with an emphasis on chemosensory systemsNucleic Acids Research, 2019
- Unraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putidaScientific Reports, 2019
- PseudomonasPublished by Wiley ,2015
- Hierarchical management of carbon sources is regulated similarly by the CbrA/B systems in Pseudomonas aeruginosa and Pseudomonas putidaMicrobiology, 2014
- Pseudomonasgenomes: diverse and adaptableFEMS Microbiology Reviews, 2011
- Dual Involvement of CbrAB and NtrBC in the Regulation of Histidine Utilization inPseudomonas fluorescensSBW25Genetics, 2008
- The Role of Two-Component Regulation Systems in the physiology of the Bacterial CellScience Progress, 2006
- The CbrA–CbrB two‐component regulatory system controls the utilization of multiple carbon and nitrogen sources in Pseudomonas aeruginosaMolecular Microbiology, 2001
- Cell signalling by oligosaccharides. Two-component systems in Pseudomonas aeruginosa: why so many?Trends in Microbiology, 2000