Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) Analysis for the Identification of Pathogenic Microorganisms: A Review
Open Access
- 18 July 2021
- journal article
- review article
- Published by MDPI AG in Microorganisms
- Vol. 9 (7), 1536
- https://doi.org/10.3390/microorganisms9071536
Abstract
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used in the field of clinical microbiology since 2010. Compared with the traditional technique of biochemical identification, MALDI-TOF MS has many advantages, including convenience, speed, accuracy, and low cost. The accuracy and speed of identification using MALDI-TOF MS have been increasing with the development of sample preparation, database enrichment, and algorithm optimization. MALDI-TOF MS has shown promising results in identifying cultured colonies and rapidly detecting samples. MALDI-TOF MS has critical research applications for the rapid detection of highly virulent and drug-resistant pathogens. Here we present a scientific review that evaluates the performance of MALDI-TOF MS in identifying clinical pathogenic microorganisms. MALDI-TOF MS is a promising tool in identifying clinical microorganisms, although some aspects still require improvement.Funding Information
- Special Foundation for National Science and Technology Basic Research Program of China (2019FY101200, Z181100001618015, No. ZK201000)
- National Natural Science Foundation of China (81971979 and 82002178)
- Fundamental Research Funds for the Central Universities (3332020005)
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