Insights into neutralizing antibody responses in individuals exposed to SARS-CoV-2 in Chile
Open Access
- 12 February 2021
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science Advances
- Vol. 7 (7), eabe6855
- https://doi.org/10.1126/sciadv.abe6855
Abstract
Chile has one of the worst numbers worldwide in terms of SARS-CoV-2 positive cases and COVID-19–related deaths per million inhabitants; thus, characterization of neutralizing antibody (NAb) responses in the general population is critical to understanding of immunity at the local level. Given our inability to perform massive classical neutralization assays due to the scarce availability of BSL-3 facilities in the country, we developed and fully characterized an HIV-based SARS-CoV-2 pseudotype, which was used in a 96-well plate format to investigate NAb responses in samples from individuals exposed to SARS-CoV-2 or treated with convalescent plasma. We also identified samples with decreased or enhanced neutralization activity against the D614G spike variant compared with the wild type, indicating the relevance of this variant in host immunity. The data presented here represent the first insights into NAb responses in individuals from Chile, serving as a guide for future studies in the country.Funding Information
- Fondo Nacional de Desarrollo Científico y Tecnológico (1190156)
- Fondo Nacional de Desarrollo Científico y Tecnológico (1180798)
- Fondo Nacional de Desarrollo Científico y Tecnológico (1171570)
- Universidad de Chile (UCH-1566)
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