Prognostic Relevance of HJURP Expression in Patients with Surgically Resected Colorectal Cancer
Open Access
- 26 October 2020
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 21 (21), 7928
- https://doi.org/10.3390/ijms21217928
Abstract
HJURP is a key factor for CENP-A deposition and maintenance in centromeres. The role of mis-regulation of histone chaperones in cancer initiation and progression has been studied. However, its role in colorectal cancer is still unclear. In this study, we aimed to evaluate the expression of HJURP in 162 colorectal cancer tissue. To investigate the function of HJURP in the colorectal cancer cell, we suppressed HJURP expression by siRNA and confirmed proliferation, migration, invasion, and anchorage independent of colony forming ability. The association between HJURP expression levels and clinicopathological factors was evaluated in 162 CRC tissues using immunohistochemistry. The overall survival rate in patients of HJURP high expression was higher than those in HJURP low expression in CRC. Suppressing HJURP expression decreased cellular proliferation, invasion, and migration in four CRC cell lines: HT29, HCT116, SW480, SW620 in vitro study. Our findings revealed that the knockdown of HJURP suppressed the proliferation, migration, invasion, and tumorigenicity in CRC cells. Due to its strong association with CRC, HJURP could be a potential prognostic biomarker and a novel target for drug discovery.This publication has 23 references indexed in Scilit:
- Cetuximab Plus Irinotecan, Fluorouracil, and Leucovorin As First-Line Treatment for Metastatic Colorectal Cancer: Updated Analysis of Overall Survival According to Tumor KRAS and BRAF Mutation StatusJournal of Clinical Oncology, 2011
- Vertebrate kinetochore protein architecture: protein copy numberThe Journal of cell biology, 2010
- The expression level of HJURP has an independent prognostic impact and predicts the sensitivity to radiotherapy in breast cancerBreast Cancer Research, 2010
- Advanced colorectal cancer: ESMO Clinical Recommendations for diagnosis, treatment and follow-upAnnals of Oncology, 2009
- HJURP Is a Cell-Cycle-Dependent Maintenance and Deposition Factor of CENP-A at CentromeresCell, 2009
- Centromere-Specific Assembly of CENP-A Nucleosomes Is Mediated by HJURPCell, 2009
- Activation of Holliday Junction–Recognizing Protein Involved in the Chromosomal Stability and Immortality of Cancer CellsCancer Research, 2007
- Survival of Patients With Advanced Colorectal Cancer Improves With the Availability of Fluorouracil-Leucovorin, Irinotecan, and Oxaliplatin in the Course of TreatmentJournal of Clinical Oncology, 2004
- FOLFIRI Followed by FOLFOX6 or the Reverse Sequence in Advanced Colorectal Cancer: A Randomized GERCOR StudyJournal of Clinical Oncology, 2004