Photosynthetic Reduction of Xylose to Xylitol Using Cyanobacteria
- 1 June 2020
- journal article
- research article
- Published by Wiley in Biotechnology Journal
- Vol. 15 (6), e1900354
- https://doi.org/10.1002/biot.201900354
Abstract
Photosynthetic generation of reducing power makes cyanobacteria an attractive host for biochemical reduction compared to cell-free and heterotrophic systems, which require burning of additional resources for the supply of reducing equivalent. Here, using xylitol synthesis as an example, efficient uptake and reduction of xylose photoautotrophically in Synechococcus elongatus PCC7942 are demonstrated upon introduction of an effective xylose transporter from Escherichia coli (Ec-XylE) and the NADPH-dependent xylose reductase from Candida boidinii (Cb-XR). Simultaneous activation of xylose uptake and matching of cofactor specificity enabled an average xylitol yield of 0.9 g g(-1) xylose and a maximum productivity of about 0.15 g L-1 day(-1) OD-1 with increased level of xylose supply. While long-term cellular maintenance still appears challenging, high-density conversion of xylose to xylitol using concentrated resting cell further pushes the titer of xylitol formation to 33 g L-1 in six days with 85% of maximum theoretical yield. While the results show that the unknown dissipation of xylose can be minimized when coupled to a strong reaction outlet, it remains to be the major hurdle hampering the yield despite the reported inability of cyanobacteria to metabolize xylose.Keywords
This publication has 35 references indexed in Scilit:
- Photoautotrophic synthesis of butyrate by metabolically engineered cyanobacteriaBiotechnology & Bioengineering, 2018
- A balanced ATP driving force module for enhancing photosynthetic biosynthesis of 3-hydroxybutyrate from CO2Metabolic Engineering, 2018
- Versatility of hydrocarbon production in cyanobacteriaApplied Microbiology and Biotechnology, 2016
- Introducing extra NADPH consumption ability significantly increases the photosynthetic efficiency and biomass production of cyanobacteriaMetabolic Engineering, 2016
- Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathwayMetabolic Engineering, 2016
- Synthesis of 2,3-butanediol by Synechocystis sp. PCC6803 via heterologous expression of a catabolic pathway from lactic acid- and enterobacteriaMetabolic Engineering, 2013
- Cyanobacterial conversion of carbon dioxide to 2,3-butanediolProceedings of the National Academy of Sciences of the United States of America, 2013
- Engineering a cyanobacterium as the catalyst for the photosynthetic conversion of CO2 to 1,2-propanediolMicrobial Cell Factories, 2013
- Photosynthetic production of 2-methyl-1-butanol from CO2 in cyanobacterium Synechococcus elongatus PCC7942 and characterization of the native acetohydroxyacid synthaseEnergy & Environmental Science, 2012
- D-XylitolPublished by Springer Science and Business Media LLC ,2012