Effects of Sodium Nitroprusside on Salt Stress Tolerance of Tocopherol-Deficient Arabidopsis Thaliana Plants

Abstract

In the present study, effects of exogenous sodium nitroprusside (SNP), a nitric oxide(•NO) donor, on lipid peroxidation and antioxidant enzyme activities in wild type and tocopheroldeficient lines vte1 and vte4 of Arabidopsis thaliana subjected to 200 mM NaCl were studied. In wildtype plants, pretreatment with SNP did not change level of thiobarbituric acid reactive substances(TBARS), but decreased the activities of dehydroascorbate reductase and guaiacol peroxidaseunder salt stress. In mutant line vte1, which lacks all forms of tocopherols, pretreatment with SNPreduced TBARS level and increases the activities of glutathione reductase and guaiacol peroxidaseunder salt stress. Ascorbate peroxidase activity decreased under salt stress conditions in bothmutant lines, pretreated with SNP. It can be concluded, that pretreatment with SNP couldattenuate salt-induced injuries in A. thaliana plants via up-regulation of activity of antioxidantenzymes and attenuate lipid peroxidation