Biocontrol Efficacy of Bacillus cereus Strain Bc-cm103 Against Meloidogyne incognita
- 1 August 2021
- journal article
- research article
- Published by Scientific Societies in Plant Disease
- Vol. 105 (8), 2061-2070
- https://doi.org/10.1094/pdis-03-20-0648-re
Abstract
Root-knot nematodes (Meloidogyne spp.) are soilborne pathogens that infect vegetable crops and cause major economic losses worldwide annually. Therefore, there is an urgent need for novel nematicides or biological control agents to reduce the damage caused by root-knot nematodes. In this study, we tested efficacy of the Bacillus cereus strain Bc-cm103, isolated from the rhizoplane of Cucumis metuliferus, against Meloidogyne incognita. Strain Bc-cm103 fermentation broth caused 100% mortality of the nematode second-stage juveniles within 12 h and decreased the egg hatching rate by 40.06% within 72 h compared with sterile water. Confocal laser-scanning microscopy revealed that strain Bc-cm103 formed a biofilm on cucumber (C. sativus) roots, which protected the roots from the infection of M. incognita. Additionally, strain Bc-cm103 activated the defense-responsive genes PR1, PR2, LOX1, and CTR1 in cucumber. Furthermore, strain Bc-cm103 significantly reduced the appearance of root galls in pot, split-root, and field tests. These results indicated that B. cereus strain Bc-cm103 had a strong suppressive effect on M. incognita and therefore could be used as a potential biocontrol agent against this pathogen.Keywords
Funding Information
- National Key R&D Program (2017YFD0200600, 2016YFD0201000)
- National Natural Science Foundation of China (31672010, 31871942)
- China Agriculture Research System (CARS-25)
- Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS)
This publication has 43 references indexed in Scilit:
- Development of a real‐time PCR assay for Pseudomonas cichorii, the causal agent of midrib rot in greenhouse‐grown lettuce, and its detection in irrigating waterPlant Pathology, 2010
- Current and future management strategies in intensive crop production systems.Published by CABI Publishing ,2009
- Red Light‐induced Systemic Disease Resistance against Root‐knot Nematode Meloidogyne javanica and Pseudomonas syringae pv. tomato DC 3000Journal of Phytopathology, 2008
- Intercellular communication in plants: evidence for two rapidly transmitted systemic signals generated in response to electromagnetic field stimulation in tomatoPlant, Cell & Environment, 2007
- Involvement of Jasmonic Acid/Ethylene Signaling Pathway in the Systemic Resistance Induced in Cucumber by Trichoderma asperellum T203Phytopathology®, 2005
- Evaluation of chemical and integrated strategies as alternatives to methyl bromide for the control of root‐knot nematodes in GreecePest Management Science, 2003
- Rhizobacteria-mediated induced systemic resistance (ISR) in Arabidopsis requires sensitivity to jasmonate and ethylene but is not accompanied by an increase in their productionPhysiological and Molecular Plant Pathology, 2000
- Rhizosphere Interactions and the Exploitation of Microbial Agents for the Biological Control of Plant-Parasitic NematodesAnnual Review of Phytopathology, 2000
- Control of the rootknot nematodemeloidogyne javanicabyBacillus cereusBiocontrol Science and Technology, 1993
- Wirkung der pflanzengesundheitsfördernden Rhizobakterien Agrobacterium radiobacter und Bacillus sphaerkus auf den Globodera pallida-Befall der Kartoffel und das PflanzenwachstumJournal of Phytopathology, 1992