Host Plant Adaptation Drives Changes inDiaphorina citriProteome Regulation, Proteoform Expression, and Transmission of ‘CandidatusLiberibacter asiaticus’, the Citrus Greening Pathogen
- 1 January 2022
- journal article
- research article
- Published by Scientific Societies in Phytopathology®
- Vol. 112 (1), 101-115
- https://doi.org/10.1094/phyto-06-21-0275-r
Abstract
The Asian citrus psyllid (Diaphorina citri) is a pest of citrus and the primary insect vector of the bacterial pathogen, ‘Candidatus Liberibacter asiaticus’ (CLas), which is associated with citrus greening disease. The citrus relative Murraya paniculata (orange jasmine) is a host plant of D. citri but is more resistant to CLas compared with all tested Citrus genotypes. The effect of host switching of D. citri between Citrus medica (citron) and M. paniculata plants on the acquisition and transmission of CLas was investigated. The psyllid CLas titer and the proportion of CLas-infected psyllids decreased in the generations after transfer from CLas-infected citron to healthy M. paniculata plants. Furthermore, after several generations of feeding on M. paniculata, pathogen acquisition (20 to 40% reduction) and transmission rates (15 to 20% reduction) in psyllids transferred to CLas-infected citron were reduced compared with psyllids continually maintained on infected citron. Top-down (difference gel electrophoresis) and bottom-up (shotgun MS/MS) proteomics methods were used to identify changes in D. citri protein expression resulting from host plant switching between Citrus macrophylla and M. paniculata. Changes in expression of insect metabolism, immunity, and cytoskeleton proteins were associated with host plant switching. Both transient and sustained feeding on M. paniculata induced distinct patterns of protein expression in D. citri compared with psyllids reared on C. macrophylla. The results point to complex interactions that affect vector competence and may lead to strategies to control the spread of citrus greening disease.Keywords
Funding Information
- Agricultural Research Service (8062-22410-007-000-D)
- National Institute of Food and Agriculture (2016-70016-24779)
- California Citrus Research Board (5300-163)
This publication has 56 references indexed in Scilit:
- Cleaning up the masses: Exclusion lists to reduce contamination with HPLC-MS/MSJournal of Proteomics, 2013
- Total kinetic analysis reveals how combinatorial methylation patterns are established on lysines 27 and 36 of histone H3Proceedings of the National Academy of Sciences of the United States of America, 2012
- Biomarker discovery from the top down: Protein biomarkers for efficient virus transmission by insects (Homoptera: Aphididae) discovered by coupling genetics and 2‐D DIGEProteomics, 2011
- Murraya paniculata and Related Species as Potential Hosts and Inoculum Reservoirs of ‘Candidatus Liberibacter asiaticus’, Causal Agent of HuanglongbingPlant Disease, 2010
- Structural cuticular proteins from arthropods: Annotation, nomenclature, and sequence characteristics in the genomics eraInsect Biochemistry and Molecular Biology, 2010
- Differences in heat shock protein 70 expression during larval and early spat development in the Eastern oyster, Crassostrea virginica (Gmelin, 1791)Cell Stress and Chaperones, 2009
- Development of an integrated approach for evaluation of 2‐D gel image analysis: Impact of multiple proteins in single spots on comparative proteomics in conventional 2‐D gel/MALDI workflowElectrophoresis, 2007
- Three different DnaK proteins are functionally expressed in the cyanobacterium Synechocystis sp. PCC 6803Microbiology, 2007
- PGRP-SB1: An N-acetylmuramoyl l-alanine amidase with antibacterial activityBiochemical and Biophysical Research Communications, 2006
- Structure and expression of a Manduca sexta larval cuticle gene homologous to Drosophila cuticle genesJournal of Molecular Biology, 1988