Effect of nanocapsules and extract of Metarhizium anisopliae in inhibiting acetylcholine esterase enzyme in Musca domestica larvae.
Open Access
- 20 May 2023
- journal article
- research article
- Published by College of Science for Women in Baghdad Science Journal
- Vol. 21 (1), 41-52
- https://doi.org/10.21123/bsj.2023.7900
Abstract
M. domestica is the most important insect that transmit pathogens for diseases in the world. The use of nanotechnology is eco-friendly method in control pests. The study aims to investigate the feasibility of bio-manufacturing nanocapsules of fungal secondary metabolites in order to improve the efficiency of metabolite and assess their inhibitory effect on the acetylcholine esterase enzyme in housefly larvae. An equal mixture of organic solvents, ethyl acetate and dichloromethane, was used to extract the metabolic products of the fungus M. anisopliae, (PEG4000) and chitosan was used in the preparation of nanocapsules. The results of the DLS granular size assay showed that the size of the extract particles and the size of the chitosan and (PEG 4000) nanocapsules were 610, 217 and 188 nm, respectively. The SEM images showed that the diameter of the extract and the nanocapsules chitosan and polyethylene glycol 4000 reached a rate 547.5, 17.8 and 26.2 nm, respectively. The FTIR showed that the extract of the second products of the fungus contains functional groups like: alkynes and alkenes, amines, carboxyl and aromatic groups, while the presence of groups of phenols, alcohol, amines, alkenes, and alkyl halides was recorded for nanocapsules of chitosan and PEG. The results showed that the extract of fungal metabolic and nanocapsules has an inhibitory effect on acetylcholinesterase enzyme and reached the highest inhibition rate 53.2 ,36.3,18.2% when treated with nanocapsules PEG at a concentration 500 ppm, extract of fungal metabolites at a concentration 50,000 ppm, chitosan nanocapsules at a concentration 500 ppm respectively. It is clear that acetylcholinesterase inhibition is one of the mechanisms of fungi metabolic action and the nanocapsules prepared from them.This publication has 10 references indexed in Scilit:
- Flow Injection Analysis with Turbidity Detection for the Quantitative Determination of Mebeverine Hydrochloride in Pharmaceutical FormulationsBaghdad Science Journal, 2022
- Development and Validation CFIA / MZ System as a Green Method for Determination of Thiol Drug (D-PEN)Egyptian Journal of Chemistry, 2021
- Capillary zone electrophoresis as a quality assessment tool of paracetamol and phenylephrine hydrochloride in presence of paracetamol impuritiesTURKISH JOURNAL OF CHEMISTRY, 2021
- Spectrophotometric Determination of Cu (+II) by Complexation with 2-(4-biphenyl) Imidazo [1,2-] Pyrimidine-3-Hydrazone and Studying Characteristics of prepared complex.Egyptian Journal of Chemistry, 2020
- Characteristics of internal medicine residents who successfully match into cardiology fellowshipsBMC Medical Education, 2020
- Analytical investigation of ternary mixture of phenylephrine hydrochloride, dimetindene maleate and benzalkonium chloride using validated stability indicating HPLC-DAD methodDrug Development and Industrial Pharmacy, 2020
- Determination of Ibuprofen and Phenylephrine in Tablets by High-Performance Thin Layer Chromatography and in Plasma by High-Performance Liquid Chromatography with Diode Array DetectionJournal of Chromatographic Science, 2019
- Application of derivative emission fluorescence spectroscopy for determination of ibuprofen and phenylephrine simultaneously in tablets and biological fluidsSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2018
- What treatments are effective for common cold in adults and children?BMJ, 2018
- The Quantitative Detection of Phenylephrine in Pharmaceutical Preparations and Spiked Human Urine by VoltammetryRussian Journal of Electrochemistry, 2018