Corrigendum: Priming Is Dispensable for NLRP3 Inflammasome Activation in Human Monocytes In Vitro

Abstract

A Corrigendum on Priming Is Dispensable for NLRP3 Inflammasome Activation in Human Monocytes In Vitro By Gritsenko A, Yu S, Martin-Sanchez F, Diaz-del-Olmo I, Nichols E-M, Davis DM, Brough D and Lopez-Castejon G (2020). Front. Immunol. 11:565924. doi: 10.3389/fimmu.2020.565924 In the original article, there was an error in the section Experimental Procedures, Cell Culture and Treatments, Paragraph 2. The incorrect guide RNA oligonucleotide sequences were given for GSDMD knockout in THP-1 cells: “GSDMD knockout THP-1 cells were lentivirally generated using guide RNA oligonucleotides sequence 5′-CACCGCTGCAAGCTGGCCAGGTACC-3′ and 5′ AAACGGTACCTGGCCAGCTTGCAGC-3′ (22) by utilizing the lentiCRISPR v2 plasmid system. lentiCRISPR v2 was a gift from Feng Zhang (Addgene plasmid # 52961; http://n2t.net/addgene:52961; RRID : Addgene_52961)”. A correction has been made to the above sentence, as follows: “GSDMD knockout THP-1 cells were lentivirally generated using guide RNA oligonucleotide sequences 5’-CACCGACCAGCCTGCAGAGCTCCAC-3’ and 5’-AAACGTGGAGCTCTGCAGGCTGGTC-3’ (22) by utilizing the lentiCRISPR v2 plasmid system. lentiCRISPR v2 was a gift from Feng Zhang (Addgene plasmid #52961; http://n2t.net/addgene:52961; RRID : Addgene_52961)”. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated. All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher. Keywords: macrophage, inflammasome, NLRP3, priming, monocytes, IL-18, GSDMD Citation: Gritsenko A, Yu S, Martin-Sanchez F, Diaz-del-Olmo I, Nichols E-M, Davis DM, Brough D and Lopez-Castejon G (2021) Corrigendum: Priming Is Dispensable for NLRP3 Inflammasome Activation in Human Monocytes In Vitro. Front. Immunol. 12:763899. doi: 10.3389/fimmu.2021.763899 Received: 24 August 2021; Accepted: 27 August 2021; Published: 13 September 2021. Edited and reviewed by: Copyright © 2021 Gritsenko, Yu, Martin-Sanchez, Diaz-del-Olmo, Nichols, Davis, Brough and Lopez-Castejon. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. *Correspondence: Gloria Lopez-Castejon, gloria.lopez-castejon@manchester.ac.uk