Analytical evaluation of three soluble transferrin receptor measurement systems for diagnosis of iron deficiency anemia: A retrospective study
Open Access
- 1 August 2020
- journal article
- research article
- Published by Wiley in Journal of Clinical Laboratory Analysis
- Vol. 34 (8), e23342
- https://doi.org/10.1002/jcla.23342
Abstract
Background Soluble transferrin receptor (sTfR) is a promising indicator of iron deficiency anemia (IDA). Here, we investigated the application value of sTfR assays based on three different methods for the diagnosis of IDA. Methods The sTfR concentrations in two groups of patient specimens with high-level and low-level sTfR concentrations and in quality control materials were measured four times a day for five consecutive days to evaluate the precision of the three methods. We selected patients with IDA, anemia of chronic disease (ACD), or chronic diseases with iron deficiency anemia (CIDA), and apparently healthy subjects, and measured the serum sTfR concentrations in all subjects using the three different methods. The cutoff points for an IDA diagnosis using the three assays and their corresponding clinical sensitivities and specificities were calculated by receiver operating characteristic analysis. Results For the diagnosis of IDA, the cutoff points of sTfR measured by the chemiluminescent, immunoturbidimetric, and immunonephelometric assays were 2.91, 6.70, and 2.48 mg/L, respectively. The corresponding sensitivities were 85.59%, 85.59%, and 85.59%, the specificities were 91.47%, 90.31%, and 90.70%, and area under the curve was 0.943, 0.944, and 0.936, respectively. The sTfR concentrations measured by the different methods were significantly higher in the IDA and CIDA groups than in the other two groups (P < .05). Conclusions The sTfR based on the three different measurement methods presented promising analytical performances and met the clinical requirements for sensitivity and specificity. However, the different measurement methods had markedly different cutoff points for IDA diagnosis, which should be critically considered in clinical practice.This publication has 21 references indexed in Scilit:
- Evaluation of serum transferrin receptor and sTfR ferritin indices in diagnosing and differentiating iron deficiency anemia from anemia of chronic diseaseIndian Journal of Pediatrics, 2010
- Worldwide prevalence of anaemia, WHO Vitamin and Mineral Nutrition Information System, 1993–2005Public Health Nutrition, 2008
- Anemia of Chronic DiseaseThe New England Journal of Medicine, 2005
- High serum transferrin receptor level in anemia of chronic disorders indicates coexistent iron deficiencyAmerican Journal of Hematology, 2003
- Serum soluble transferrin receptor concentration is an accurate estimate of the mass of tissue receptorsExperimental Hematology, 2001
- The cellular mechanisms of body iron homeostasisBiological Research, 2000
- Circulating transferrin receptors and assessment of iron statusThe Journal of Nutritional Biochemistry, 1994
- Serum Transferrin ReceptorAnnual Review of Medicine, 1993
- Inhibition of human erythroid colony-forming units by tumor necrosis factor requires beta interferon.JCI Insight, 1993
- NEW TOOLS FOR CLINICAL EVALUATION OF ERYTHRON FUNCTION IN MANBritish Journal of Haematology, 1992