Interaction between Polyphenolic Antioxidants and Saccharomyces cerevisiae Cells Defective in Heavy Metal Transport across the Plasma Membrane
Open Access
- 4 November 2020
- journal article
- research article
- Published by MDPI AG in Biomolecules
- Vol. 10 (11), 1512
- https://doi.org/10.3390/biom10111512
Abstract
Natural polyphenols are compounds with important biological implications which include antioxidant and metal-chelating characteristics relevant for their antimicrobial, antitumor, or antiaging potential. The mechanisms linking polyphenols and heavy metals in their concerted actions on cells are not completely elucidated. In this study, we used the model eukaryotic microorganism Saccharomyces cerevisiae to detect the action of widely prevalent natural polyphenols on yeast cells defective in the main components involved in essential heavy metal transport across the plasma membrane. We found that caffeic and gallic acids interfered with Zn accumulation, causing delays in cell growth that were alleviated by Zn supplementation. The flavones morin and quercetin interfered with both Mn and Zn accumulation, which resulted in growth improvement, but supplemental Mn and especially Zn turned the initially benefic action of morin and quercetin into potential toxicity. Our results imply that caution is needed when administering food supplements or nutraceuticals which contain both natural polyphenols and essential elements, especially zinc.This publication has 73 references indexed in Scilit:
- The Neglected Significance of “Antioxidative Stress”Oxidative Medicine and Cellular Longevity, 2012
- Curcumin Inhibits Growth of Saccharomyces cerevisiae through Iron ChelationEukaryotic Cell, 2011
- Zn- and Cu-thioneins: a functional classification for metallothioneins?JBIC Journal of Biological Inorganic Chemistry, 2011
- Roles of Two Activation Domains in Zap1 in the Response to Zinc Deficiency in Saccharomyces cerevisiaeOnline Journal of Public Health Informatics, 2011
- Differential control of Zap1-regulated genes in response to zinc deficiency in Saccharomyces cerevisiaeBMC Genomics, 2008
- Role ofL-Histidine in Conferring Tolerance to Ni2+inSacchromyces cerevisiaeCellsBioscience, Biotechnology, and Biochemistry, 2005
- Getting started with yeastMethods in Enzymology, 2002
- Involvement of Thioredoxin Peroxidase Type II (Ahp1p) ofSaccharomyces cerevisiaein Mn2+HomeostasisBioscience, Biotechnology, and Biochemistry, 1999
- The Gene Encodes the Low Affinity Zinc Transporter inOnline Journal of Public Health Informatics, 1996
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976