α-Mangostin protects against high-glucose induced apoptosis of human umbilical vein endothelial cells
Open Access
- 12 December 2017
- journal article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 37 (6)
- https://doi.org/10.1042/bsr20170779
Abstract
Diabetic vascular complications result from high-glucose induced vascular endothelial cell dysfunction. There is an emerging need for novel drugs with vascular endothelial cell protective effects for the treatment of diabetic vascular complications. The present study aimed to investigate the protective effect of α-mangostin against high-glucose induced apoptosis of cultured human umbilical vein endothelial cells (HUVECs). HUVECs were treated with glucose to induce apoptosis. The expression of the apoptosis-related proteins, Bcl-2, Bax, and cleaved caspase-3, were detected by Western blotting. Ceramide concentration and acid sphingomyelinase (ASM) activity were assayed by HPLC. The cell apoptosis rate was detected by flow cytometry after staining with annexin V/propidium iodide (PI). Compared with HUVECs cultured in 5 mM glucose, cells cultured in 30 mM glucose exhibited a higher apoptosis rate, up-regulation of cleaved caspase-3 and Bax (proapoptotic proteins), down-regulation of Bcl-2 (anti-apoptotic protein), increased ceramide concentration, and enhanced ASM activity (all P<0.05). α-Mangostin (15 µM) significantly attenuated the high-glucose induced increase in apoptosis rate (8.64 ± 2.16 compared with 19.6 ± 3.54%), up-regulation of cleaved caspase-3 and Bax, down-regulation of Bcl-2, elevation of ceramide level, and enhancement of ASM activity (all P<0.05). The effects of desipramine were similar to those of α-mangostin. The protective effect of α-mangostin on high-glucose induced apoptotic damage may be mediated by an inhibition of ASM and thus a decreased level of ceramide.Keywords
This publication has 47 references indexed in Scilit:
- Antiangiogenic antitumor activities of IGFBP-3 are mediated by IGF-independent suppression of Erk1/2 activation and Egr-1–mediated transcriptional eventsBlood, 2011
- Endothelial cell substrate stiffness influences neutrophil transmigration via myosin light chain kinase-dependent cell contractionBlood, 2011
- Zinc finger protein A20 overexpression inhibits monocyte homing and protects endothelial cells from injury induced by high glucoseEvolution, 2011
- Evaluation of Mangosteen juice blend on biomarkers of inflammation in obese subjects: a pilot, dose finding studyNutrition Journal, 2009
- Plasma Ceramides Are Elevated in Obese Subjects With Type 2 Diabetes and Correlate With the Severity of Insulin ResistanceDiabetes, 2009
- Anti-inflammatory activity of mangostins from Garcinia mangostanaFood and Chemical Toxicology, 2008
- Endothelial Dysfunction in Normal and Abnormal Glucose MetabolismPublished by S. Karger AG ,2008
- Phenolics from hull of Garcinia mangostana fruit and their antioxidant activitiesFood Chemistry, 2007
- Secretory Zn2+-dependent Sphingomyelinase Activity in the Serum of Patients with Type 2 Diabetes is ElevatedHormone and Metabolic Research, 2003
- Elevated glucose impairs endothelium-dependent relaxation by activating protein kinase C.JCI Insight, 1991