Persons in senior administrative positions (SAP) in public universities are an important segment of the workforce, which could play an important role in the control of HIV/AIDS through their impact on the attitude to people living with HIV/AIDS (PLWHA) both within and outside the university community. However, there is a gross dearth of information on their perception of HIV/AIDS and the corresponding attitude to PLWHA. This is unlike the profusion of data on the perception of students in tertiary institutions of higher learning. The objective of this study was thus to determine the level of awareness and perception of HIV/AIDS, and the workplace attitude to PLWHA, of persons in SAP in public universities and the association with the demographic factors of age, gender and religion. A self-administered questionnaire, ‘Awareness and Attitude towards PLWHA scale’ was used to elicit responses from 250 persons, representing 91.6% of those eligible, in a cross-sectional survey of Deans of Faculties, Directors and Heads of Departments in two public universities in Edo State, Nigeria. Mean scores and mean percentage responses were compared between groups using Student’s t-test and one-way ANOVA. The level of significance was set at P <0.05. The mean (standard deviation, SD) of Respondents with correct answers to the questions on perception was 72.7 (12.8)% on perception of the nature of HIV/AIDS, 45.1 (38.6)% on transmission, 90.9 (7.4)% on diagnosis and 85.8 (10.8)% on perception of its prevention (P = 0.034). The mean (SD) of the responses on general attitude was 96.8 (2.9)% and that on workplace attitude 85.2 (7.5)% (P = 0.036). There was no statistically significant difference between the mean overall scores on perception and attitude in relation to age, gender and religion. We conclude that the perception of HIV/AIDS and workplace attitude to PLWHA among persons in SAP in public universities in Nigeria are quite favourable, except in relation to the transmission of infection. We also conclude that the perception is unrelated to the demographic factors of age, gender and religion. These findings are relevant to the development of HIV/AIDS control messages, particularly those targeted at the reduction of stigmatization and discrimination, important attitudinal problems fueling the transmission of HIV/AIDS in resource-poor countries.