Dlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme
Open Access
- 22 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 11 (1), 1-15
- https://doi.org/10.1038/s41598-021-81434-x
Abstract
Neural crest cells (NCCs) give rise to various tissues including neurons, pigment cells, bone and cartilage in the head. Distal-less homeobox 5 (Dlx5) is involved in both jaw patterning and differentiation of NCC-derivatives. In this study, we investigated the differentiation potential of head mesenchyme by forcing Dlx5 to be expressed in mouse NCC (NCCDlx5). In NCCDlx5 mice, differentiation of dermis and pigment cells were enhanced with ectopic cartilage (ec) and heterotopic bone (hb) in different layers at the cranial vertex. The ec and hb were derived from the early migrating mesenchyme (EMM), the non-skeletogenic cell population located above skeletogenic supraorbital mesenchyme (SOM). The ec developed within Foxc1+-dura mater with increased PDGFRα signalling, and the hb formed with upregulation of BMP and WNT/β-catenin signallings in Dermo1+-dermal layer from E11.5. Since dermal cells express Runx2 and Msx2 in the control, osteogenic potential in dermal cells seemed to be inhibited by an anti-osteogenic function of Msx2 in normal context. We propose that, after the non-skeletogenic commitment, the EMM is divided into dermis and meninges by E11.5 in normal development. Two distinct responses of the EMM, chondrogenesis and osteogenesis, to Dlx5-augmentation in the NCCDlx5 strongly support this idea.Funding Information
- Ministry of Education, Culture, Sports, Science and Technology (18K06821)
- Ministry of Education, Culture, Sports, Science, and Technology of Japan (18K19605)
This publication has 78 references indexed in Scilit:
- Prevention of Premature Fusion of Calvarial Suture in GLI-Kruppel Family Member 3 (Gli3)-deficient Mice by Removing One Allele of Runt-related Transcription Factor 2 (Runx2)Online Journal of Public Health Informatics, 2012
- Regulation of cranial morphogenesis and cell fate at the neural crest-mesoderm boundary by engrailed 1Development, 2012
- Role of canonical Wnt signaling/β-catenin viaDermo1in cranial dermal cell developmentDevelopment, 2010
- Inactivation of Msx1 and Msx2 in neural crest reveals an unexpected role in suppressing heterotopic bone formation in the headDevelopmental Biology, 2010
- EphA4 as an effector of Twist1 in the guidance of osteogenic precursor cells during calvarial bone growth and in craniosynostosisDevelopment, 2009
- Stripes and belly-spots—A review of pigment cell morphogenesis in vertebratesSeminars in Cell & Developmental Biology, 2009
- CYCLOPS, a mediator of symbiotic intracellular accommodationProceedings of the National Academy of Sciences of the United States of America, 2008
- An endothelin-1 switch specifies maxillomandibular identityProceedings of the National Academy of Sciences of the United States of America, 2008
- Dlx5, a Positive Regulator of Osteoblastogenesis, is Essential for Osteoblast-Osteoclast CouplingThe American Journal of Pathology, 2008
- α-Synuclein Expression Levels Do Not Significantly Affect Proteasome Function and Expression in Mice and Stably Transfected PC12 Cell LinesOnline Journal of Public Health Informatics, 2004