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Serological and Molecular Based Diagnosis of Toxoplasma gondii in Galliformes by using ToxPK1 gene

Farman Ullah Khan, Nageen Hussain
Journal of Scientific Research in Medical and Biological Sciences , Volume 1, pp 116-122; doi:10.47631/jsrmbs.v1i2.58

Abstract: Purpose: The zoonosis caused by Toxoplasmagondii is known to be the third worldwide parasitic infectious disease. The living life, including human, chickens, cows and goats are at high risk because of its bulky circulation in the universe. The study was conducted to diagnoseT. gondii in Galliformes. The main objective of this study was to compare VetLine Toxoplasma with ToxPK1 gene as a marker for the detection of T. gondii. Study Design: An Experimental study. Subjects and Methods: The study subjects involved were 35 broilers in which 80% were females and 20% were males. Results: It was displayed that the female broilers have a little high rate of prevalence (29.63%) as compared to male broilers (25%). Conclusion: It was concluded that VetLine Toxoplasma in which protein A/G fixes to all IgGs subclasses from various mammalian species and the use of ToxPK1 gene as a molecular marker for the detection of T. gondiiwere100% matched. To eradicate and control this neglected zoonosis, there is an urgency need for risk factors control mechanisms; secondly specific, rapid and inexpensive diagnostic and treatment approaches.
Keywords: Protein / Treatment / Zoonosis / broilers / Toxoplasma / Galliformes / Males / gondii / ToxPK1 gene

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